Synthesis of Imidazole N-Oxides 1a–1m Method A: α-Hydroxyiminoketone 7a or 7b (1.1 mmol) and formaldimine 8 (1.0 mmol) were dissolved in glacial acetic acid (3 mL) and the mixture was stirred for 12 h at room temperature. After the concentrated aq. HCl (0.5 mL) was added dropwise...
(2) Compounds are usually dissolved in DMSO and are diluted in 25 mM Hepes buffer to get final concentration of DMSO of 0.17%. Representative final compounds of Formula (I), Examples 1,5 8, and 9 have all demonstrated positive inhibitory activity of an IC50 of >50 uM in this binding ...
DNA fragments were then recovered by vacuum centrifugation, dissolved in loading dye, denatured at 95 °C for 20 min and subjected to electrophoresis on a 6% denaturing poly- acrylamide gel. Cell culture. Human colon carcinoma-derived HT29 (WT), Caco-2 (WT), SW1463 (12C/12C), SW480 (...
Stock solutions of imidazoles (10 mg/mL) were prepared in dimethyl sulphoxide (DMSO) 5% (w/vol), while SDS was dissolved in deionized water to reach 2% (w/vol) concentration. Table 1. Reference strain and clinical isolates of Candida albicans used in this study. 2.3. Agar Diffusion ...
Silver complexes 1, 3, 4, 6, and AgNO3 were dissolved in water. Complexes 2, 5, and AgSD are DMSO-soluble. Next, all chemicals were diluted into water to make a concentration 1, 0.1, 0.01 mg/mL and as a working solution were added to the medium. After 72 h of incubation, the ...
In this method, 2-methylimidazole was dissolved in MeOH, and Zn salt was separately mixed in MeOH. The former solution was then abruptly poured into the latter solution with varying reaction times of 150, 120, and 90 min at a continuous stirring speed of 350 rpm. The resulting white ...
Compound 1 was dissolved in an appropriate amount of DMSO to attain the desired concentration. The stock solution was vortexed (MSI Minishaker. IKA Company, Osaka, JAPAN) and filter sterilized into a vial through 0.45 µm millipore filters under sterile conditions and stored at −20 ◦C ...
Compound 12 (0.20 g, 0.56 mmol), thiophene-2-carbonyl chloride (0.08 g, 0.56 mmol) was dissolved in DMF (5 mL). TEA (2 drops) was added and the mixture was stirred at room temperature overnight. Then the mixture was diluted with EA (100 mL) and washed with 30 mL ˆ 3 water,...
in dark or light conditions, led to the formation of its related substance 1,2-bis[(1-methyl-1H-imidazole-2-yl)thio]ethane, C10H14N4S2 (2a), primarily isolated in the form of dihydrochloride tetrahydrate [C10H16N4S2]Cl2·4(H2O) (2b), which crystallized in the monoclinic P21/c space...
Triazole 2a (137 mg, 0.21 mmol) was dissolved in a 1:1 mixture of anhydr. EtOH and EtOAc (4 mL), Pd(C) (10 wt. %, 13 mg) was added and the mixture was stirred in H2 atmosphere (1 bar) at rt for 72 h. The catalyst was removed by filtration and the filtrate was purified ...