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Super-resolution microscopies have become an established tool in biological research. However, imaging throughput remains a main bottleneck in acquiring large datasets required for quantitative biology. Here we describe multifocal flat illumination for f
Wide-field imaging of neural activity at a cellular resolution is a current challenge in neuroscience. To address this issue, wide-field two-photon microscopy has been developed; however, the field size is limited by the objective size. Here, we develop
(B) (Orange) Indirect cell labeling requires cells that have been genetically manipulated to express a reporter gene (green). The genetic engineering options frequently employed in reporter gene applications include viruses (e.g., lentiviruses, γ-retroviruses), gene editing, or episomal plasmids (...
iBright CL1500 and FL1500 Imaging Systems 说明书 iBright™ CL1500 and FL1500 Imaging Systems Catalog Numbers A44114 and A44115 Pub. No. 100085108 Rev. C WARNING! Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear appropriate protective eyewear, clothing,...
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Of the works that did claim sup- port for parallelism, one ran on a cluster [81], one ran on a GPU [82], and five had explicit support for multi- core CPUs [83–87]. It is possible, however, that even code that was not explicitly designed to support parallel- ism might still ...
(FSE) sequence was used and is designed for optimized gray/white matter contrast. The sequence parameters for this scan are as follows: TR = 2000 ms, echo train length = 6, echo spacing = 10 ms, with the center of k-space acquired on the fourth echo, TEeff= 42 ms,...
6) using the Nonlinear Least Squares method of the R Stats Package (version 3.3.3) in R Studio (version 1.0.136), using the port algorithm restricted to fmin ≤ 0. The calcium-bound state of Tq-Ca-FLITS did not fit to this model, therefore a model with two pKa values was ...
cAMP is a key second messenger that regulates diverse cellular functions including neural plasticity. However, the spatiotemporal dynamics of intracellular cAMP in intact organisms are largely unknown due to low sensitivity and/or brightness of current g