In the rapidly expanding fields of cellular and molecular biology, fluorescence illumination and observation is becoming one of the techniques of choice to study the localization and dynamics of proteins, organelles, and other cellular compartments, as well as a tracer of intracellular protein ...
A tutorial that uses a CellPainting assay to find segmentation parameters for larger “parent” objects (nucleus, cell, and cytoplasm) and show you ways to pull out smaller features in your image by segmenting organelles within the nuclei. You will also be shown how to use RelateObjects so...
Note that, if the cell is finally recognised as an MN, each of its parts is individually labelled according to the appearance of its organelles in each of the channels. Figure 6. Schematic representation of the Labelling (LBL) phase for each object in MagNoLia. Boxes with slanted text ...
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However, because each stitch field represents a non-rigid transformation, the composition of many stitch fields can accumulate errors and result in a high frequency field that distorts cell morphology (Fig. 4h). Fig. 4: Alignment of blocks and composition with decay. A stack of 450 mouse ...
Plant cell PNG|JPG Plant cell, no labels PNG|JPG Plant cell, no organelles PNG|JPG Chloroplast PNG|JPG Chloroplast, no labels PNG|JPG Chloroplast, with labels PNG|JPG Mitochnodria, no labels PNG|JPG Mitochondria, with labels PNG|JPG
For example, Zhang, Sun, and their collaborators are using the system to study the intercellular distribution of molecules, looking at how RNA is transported and interacts with cell organelles before being translated into proteins. “This system could have profound implications in molecular biology,”...
penetrated the cell walls of algae and the sole liverwort (a plant closely related to mosses) used in the study, causing the organelles to wrinkle and deform. For these specimens, Pegg recommends sticking to aldehyde fixatives or reducing the amount of time used in the specimen preparation ...
Co-localization of the elements calcium, potassium, sodium and magnesium with sequestering organelles has been achieved by application of two microscopy te... SE Chandra,EPW Kable,GH Morrison,... - 《Journal of Cell Science》 被引量: 238发表: 1991年 Uptake of selenate and selenite by isolated...
Specifically, we trained high fidelity, explainable, and automated computational staining and destaining algorithms to learn mappings between naturally autofluorescent pixels11 of nonstained cellular organelles and their stained counterparts. We also devised robust loss function for our machine learning ...