In the rapidly expanding fields of cellular and molecular biology, fluorescence illumination and observation is becoming one of the techniques of choice to study the localization and dynamics of proteins, organelles, and other cellular compartments, as well as a tracer of intracellular protein ...
and the rest is used for training. Compounds whose Cell Painting profiles are in the test set satisfy two criteria: (1) Have pIC50≥8 in at least one of the bioassays, (2) Have a
However, as mentioned above, we do observe that also with CellTraxx the cell centre determination is influenced by internal movements of organelles within cells, resulting in some small artificial movements. We have chosen to use Incucyte S3 phase contrast images for CellTraxx analysis. Incucyte ...
"We can go small for single-molecule imaging, or move up to study multiple organelles interacting inside a cell, or how cells interact with their environments and other cells," Betzig says. "On the biggest scale, we can look at developmental biology. I'm amazed at my ...
Overview of Cell Structure - excellent 7 min. video review *** XVIVO scientific animation *** The Higgs Boson and the Fate of the Universe - video of 9.2.14 lecture re the LHC and the Higgs Boson link to complete text link to full text link to article excerpt: Lynn Margulis's gre...
specialized organs of the cell that are only a few microns in size. In fluorescence microscopy, the sample is stained with markers of a few (usually not more than five) key molecules. These markers reveal information on the properties of the organelles, including their shape, geometry, intensit...
A tutorial that uses a CellPainting assay to find segmentation parameters for larger “parent” objects (nucleus, cell, and cytoplasm) and show you ways to pull out smaller features in your image by segmenting organelles within the nuclei. You will also be shown how to use RelateObjects so...
organelles segmentation. comparing to pixel, superpixel is a bigger granularity representation of the image. pixels inside a superpixel have similar attributes, which is in reasonable agreement with the characteristic of local clustering of neuronal em images. superpixel-based features can be regarded as...
However, because each stitch field represents a non-rigid transformation, the composition of many stitch fields can accumulate errors and result in a high frequency field that distorts cell morphology (Fig. 4h). Fig. 4: Alignment of blocks and composition with decay. A stack of 450 mouse ...
The assembled 3-D image of the cell, which looks as focused as an encyclopedia drawing, can be virtually “sliced” to expose its internal elements, such as the nucleus, genetic material and organelles. Cotte and Toy are now working in collaboration with the startup Lyncée Tec SA to deve...