Figure 1. Time-lapse imaging of wound healing. Human dermal fibroblast cells were grown to confluence and labeled with 1 μM CellTracker™ Red CMTPX Dye. A scratch wound was made in the cells with a 20 μL pipette tip (A). These representative frames f...
Human dermal fibroblast cells were grown to confluence and labeled with 1 μM CellTracker™ Red CMTPX Dye. A scratch wound was made in the cells with a 20 μL pipette tip (A). These representative frames from time-lapse imaging (sample...
Finally, fluorescent labeling can lead to immunogenicity and introduction of xenobiotic compounds, such that human cells including human induced pluripotent stem cells (hiPSCs)12 and chimeric antigen receptor T (CAR-T) cells13 cannot be fluorescently labeled before in vivo use as cell therapies14. To...
The Human Glucokinase Gene β-Cell-Type Promoter: An Essential Role of Insulin Promoter Factor 1/PDX-1 in Its Activation in HIT-T15 Cells The glycolytic enzyme glucokinase plays a primary role in the glucose-responsive secretion of insulin, and defects of this enzyme can cause NIDDM. As a st...
The underlying principle of human vision and Lab color space is based on the opponent color model. Since Lab color space is based on human visual perception, Lab color space is chosen in this research. The a* channel in the Lab space denotes the relation between red and green pixel values...
short-, middle-, and long-wavelength sensitive). The three classes of cone are unequally represented in the retina, having an overall ratio of 1S:3M:6L, with some significant individual variations. As discussed later, this fact is important in understanding some features of human color vision....
Indirect Immuno-Fluorescence (IIF) microscopy imaging of human epithelial (HEp-2) cells is a popular method for diagnosing autoimmune diseases. Considering large data volumes, computer-aided diagnosis (CAD) systems, based on image-based classification, can help in terms of time, effort, and ...
A macro is a sequence of predefined commands. By recording a series of fixed operations into macros, you can improve your work efficiency. However, the disadvantage is the lack of flexibility. For example, sometimes the main steps are fixed, but the parameter tuning needs human interaction. In...
Human T Cells stained for DAPI (gray), CD3 (blue), CD4 (red), CD8 (green) and with nucleus outline (light green), cell outline (light red)Cytokit Explorer UIAfter processing an experiment, the Explorer UI application can be run within the same docker container for fast visualization of ...
Figures 3A–3E show bright-field and two-color fluorescence images of various microalgal and human cells we tested. The intelligent IACS is also able to directly detect a highly rare EpCAM (CD326)-positive circulating cell in blood from a patient with pancreatic cancer (Figure 3E). The high...