Paraformaldehyde is small molecule that penetrates cells/tissue quickly, fixing proteins and other molecules in place. Glutaraldehyde is a bigger molecule with two reactive aldehyde groups that increase the potential for fixation and help create long chains of glutaraldehyde, generating a matrix. This ...
A microscopic image recognition system for detecting a protein-based molecule by presenting a recognition image is provided. The protein-based molecule has a state of a monomer. The microscopic image recognition system includes an image capturing unit, a monomer tracking module and a texture mask. ...
Biologically produced protein drugs are generally susceptible to degradation by proteases and often exhibit immunogenicity. To address this issue, mirror-image peptide/protein binders consisting of D-amino acids have been developed so far through the mir
the spatial organizationof cellular structures and components. The assay is often used during drug screening to profile small molecules or other compounds on stained proteins in living cells. The pattern of cellular staining may revel morphological differences in samples treated by small molecule ...
Quick-freeze deep-etch replica electron microscopy combined with mica-flake technique provides high contrast, high time- and spatial-resolution images of protein molecules in solution, whose three-dimensional structure is well preserved. Thus, it might b
of a kilobase-sized mirror-image gene. We use the polymerase to encode inl-DNA an 1860 paragraph by Louis Pasteur that first proposed a mirror-image world of biology. We realize chiral steganography by embedding a chimericd-DNA/l-DNA key molecule in ad-DNA storage library, which conveys a...
RNAscope and BaseScope assays generate punctate dots each of which represents a single mRNA molecule. Overlapping mRNA molecules can lead to the signal appearing as clusters as opposed to single dots. Variations in dot sizes and color intensities can be related to differences in the number of ZZ...
a physical arrangement that is particularly effective in trapping incoming light. The pigment molecule has two essential components—a protein called opsin and a covalently linked chromophore, the latter being a derivative of vitamin A. Absorption of energy from a photon of light causes the chromophor...
Using mirror-image trypsin digestion and LC–MS/MS, we sequence a mirror-image large subunit ribosomal protein (L25) and a mirror-image Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4), and distinguish between different mutants of D-Dpo4. We also perform writing and reading of digital ...
it is a powerful tool when used correctly to obtain unbiased quantitative data. In addition, quantitative data from IHC studies can be used to increase confidence in the specificity of the IHC protocol by correlating results from IHC with other measurements of the molecule of interest, such as ...