Monoclonal Antibody Preparation: Lewis rats (6-8 week old females) were injected subcutaneously (s.c.) with 0.1 ml of protein or peptide mixed in Freund's complete adjuvant using a 25-gauge needle. Rats were boosted every 2-3 weeks with antigen and bled via the tail vein every week. Aft...
To give an injection to a calf weighing less than 500 pounds, an 18-20 gauge (g) needle with a 1 inch (2.5 cm) length is ideal. For larger animals weighing over 500 pounds, you will need a 16-18 g needle with of 1 ½ inch length. Breed can also play a part on determining ...