2.3.4 双端测序 Paired-end Sequencing双端测序大大提升了某一个DNA片段的有效测序长度,步骤如下: 合成互补链 切断模板链与flowcell引物的连接,保留互补链 与Read1测序原理类似,加入不同的测序引物(Read2 sequencing primer),测得Read2的数据 Part 3: 值得思考的问题 Q1. 二代测序读长为什么是固定的 常见illum...
These repaired cDNA fragments were 3' adenylated using Klenow Exo- (3' to 5' exo minus, Illumina). Illumina Paired-end adapters were ligated to the ends of these 3'-adenylated cDNA fragments. To select a size range of templates for downstream enrichment, the products of ligation reaction ...
Illumina,PE-121-1003,TruSeq Dual Index Sequencing Primer Box, Paired-End Illumina,20020645,BaseSpace Clarity LIMS Other Integration Perpetual License Illumina,20006576,PHI Security Package Hosted (per year) Hosted Illumina,WG-450-1013,Infinium BovineLD+ BeadChip ...
Paired End: java -jar trimmomatic-0.35.jar PE -phred33 input_forward.fq.gz input_reverse.fq.gz output_forward_paired.fq.gz output_forward_unpaired.fq.gz output_reverse_paired.fq.gz output_reverse_unpaired.fq.gz ILLUMINACLIP:TruSeq3-PE.fa:2:30:10 LEADING:3 TRAILING:3 SLIDINGWINDOW:4:15 ...
Illumina测序原理 Illumina测序原理 目录 概览制备文库(LibraryPreparation)在测序芯片上生成DNA簇(ClusterGeneration)边合成边测序(SequencingbySynthesis)双侧测序，配对测序(Paired-End,MatePair&Multiplexing)数据分析(DataAnalysis)测序工作流程概览 LibraryPreparation制备文库 ClusterGeneration生成DNA簇 Sequencing测序 Data...
Illumina文库制备概述及选择
Paired End DNA PE Adapters 5 P GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG 5 ACACTCTTTCCCTACACGACGCTCTTCCGATCT PE PCR Primer 1 0 5 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT PE PCR Primer 2 0 5 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT PE Read 1 Sequencing ...
留下 只含有反义链的 DNA 簇 Original forward strand 原始的正链 33 Part #Rev.C FOR RESEARCH USE ONLY Paired End Sequencing 双末端测序 Free 3 ends of the reverse template and lawn primers are blocked to prevent unwanted DNA priming 为了防止测序过程中不需要的 DNA 延伸, 对流动槽上结合的所有 ...
桥式结构)Paired End Sequencing双末端测序Original forward strand原始的正链Paired End Sequencing双末端测序Reverse strandTemplate反义链模板Blocked 3’-ends阻断3 ’端Sequencing primer测序引物Paired End Sequencing双末端测序Sequencing By Sy nthesis 2nd Read Read 2 边合成边测序Sequencing Paired End Libraries wi...
Can I run both single read and paired-end recipes with dual-indexed libraries? Are dual indexed libraries compatible with single end sequencing? How many indices are available with NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs) (NEB #E6440, E6442, E6444,...