Provides a checklist of the protocol steps. The checklist is intended for experienced users. Provides an interactive checklist of user-provided consumables and equipment. Provides pooling guidelines and dual indexing strategies for AmpliSeq for...
c. Specifications based on Illumina PhiX control library, or a TruSeq DNA Library created with NA12878 at supported cluster densities. Performance may vary based on library type and quality, insert size, loading concentration, and other experimental factors. ...
The checklist is intended for experienced users. Provides an interactive checklist of user-provided consumables and equipment. Provides pooling guidelines and dual indexing strategies for AmpliSeq for Illumina libraries. Provides instructions on preparing DNA fr...
3 Chapter 2 Protocol Introduction Tips and Techniques Library Prep Workflow Quantify and Dilute RNA Reverse Transcribe RNA Amplify cDNA Targets Quantify and Dilute DNA Amplify DNA Targets Transfer Amplicons Partially Digest Amplicons Ligate Indexes Clean Up ...
The checklist is intended for experienced users. Provides an interactive checklist of user-provided consumables and equipment. Provides pooling guidelines and dual indexing strategies for AmpliSeq for Illumina libraries. Provides instructions on ...
2 Chapter 2 Protocol Introduction Tips and Techniques Library Prep Workflow Quantify and Dilute DNA Amplify Targets Partially Digest Amplicons Ligate Indexes Clean Up Library Amplify Library Perform Second Cleanup Check Libraries Dilute Libraries to the Starting...