Human IL6 qPCR Primer Pair,即人IL6 qPCR引物对,主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。本引物为预先设计、经过qPCR验证、预混的引物对。 qPCR (Quantitative PCR)即定量PCR,也称实时荧光定量PCR或实时定量PCR (Real-time quantitative PCR)、实时PCR (Real-time PCR),是一种...
IL-6 qPCR Primers (3) Verified forward and reverse primers for analyzing the quantitative expression of gene. The primer mix has been verified to generate satisfactory qPCR data on Roche LightCycler480. IL-6 基本信息 IL-6 IL-6 蛋白信息 ...
意义.本文建立的RT-qPCR技术,灵敏度高,特异 性强,能精确,简便地对人IL-6mBNA进行定量,现 报道如下. 1材料与方法 1.1主要材料淋巴细胞分离液(上海生工生物 工程公司);Trizol裂解液(上海生工生物工程公 司);RT-PCR体系(大连宝生物公司);SYBRGreenI
Primers for Taqman qPCR were commercially purchased (Thermo Fisher) and listed as following: WT1: Mm01337048_m1; Nkx2.5: Mm01309813_s1; GATA4: Mm01310448_m1; TBX5: Mm00803518_m1; Tnnt2: Mm00441920_m1, Postn: Mm01284913_g1; IL-6: Mm00446190_m1; HGF: Mm01135185_m1; IGF-1: Mm...
The DNA region of interest was detected by SYBR green real-time quantitative PCR (qPCR) using primers encompassing H3K27ac and SMARCB1 enrichment locus on human STAT3 promoter determined using previously published ChIP-Seq datasets57. The sequences of the forward and reverse primers used for ...
d RT-qPCR analyzed the expression of the most often released cytokines by macrophages after IL-6 stimulation. e Elisa analysis of IL-6 showed changes in macrophage IL-6 expression over time after IL-6 stimulation. f & g Cell migration, invasion and proliferation ability of LUAD cells (A549...
Real-time PCR was performed using Hieff Qpcr SYBR Green Master Mix (Yeasen) and analyzed with a Stratagene Mx 3000P thermal cycler. Primers sequences are supplied in Supplementary Table 2. Flow cytometry analysis of hepatocytes ploidy populations Primary hepatocytes and IL6-iHPCs were fixed and ...
After DNA was purified, qPCR was used to identify the enriched genes. Primers were as follows: IL-6, forward 5′-TGCACTTTTCCCCCTAGTTG-3′ and reverse 5′-TCATGGGAAAATCCCACATT -3′; IL-6R, forward 5′-GAGGGCAGAGGCACTTACTG-3′ and reverse 5′-AGTTGCCCAACTCTTCCAGA-3′; Negative, ...
reaction with anti-SOX5 and nonspecific IgG and 10% of the pre-cleared chromatin was set aside as input control. Purified DNAs were subjected to quantitative PCR (qPCR) using the primer sets listed inSupplementary Table S3. Fold enrichment of the targeted genomic sequences were calculated over...
The cDNA products were diluted to 40 µl with sterilized ddH2O.1 µl (12.5 µg) cDNA was used for the real-time PCR reaction using 2xSG Fast qPCR Master Mix. Primers and PCR operation were according to 1.2. Real-time PCR of PTLCs PTLCs were collected and total RNA was ...