Standard curve of KE00139 AuthentiKine™ Human IL-6 ELISA Kit. ¥3200/96T Cat No.KE00139 库存:现货 Size Product Information KE00139 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL6 ELISA kit is to be used to detect and quantify protein levels of...
each well after Diluting ,(density: 6ng/L,4ng/L ,2ng/L,1ng/L,0.5ng/L) 2.add sample:Set blank wells separately (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample w...
KE00139 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL6 ELISA kit is to be used to detect and quantify protein levels of endogenous IL6. The assay recognizes human IL6. An antibody specific for IL6 has been pre-coated onto the microwells. The IL6...
2.add sample:Set blank wells separately (blank comparison wells don’t add sample andHRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution50μl to testing sample well, then addtesting sample 10μl (sample final dilutionis 5-fold),add sample to...
Western Blot, ELISA Formulation Lyophilized from a 0.2 μm filtered solution of 10 mM Hepes, 150 mM NaCl with 5% trehalose, pH 7.4. Reconstitution Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don...
www.biokanu.com大鼠白细胞介素6(IL-6)酶联免疫分析(ELISA)试剂盒使用说明书本试剂仅供研究使用目的:本试剂盒用于测定大鼠血清,组织及相关液体样本中白细胞介素6(IL-6)的含量。实验原理:本试剂盒应用双抗体夹心法测定标本中大鼠白细胞介素6(IL-6)水平。用纯化的大鼠白细胞介素6抗体包被微孔板,制成固相抗体,往包...
Two-sided statistical analysis or three-way comparison was considered statistically significant at p < 0.05. The statistical analysis was performed either in GraphPad Prism v10 for the graphs or with the R software for the heat maps. Error bars represent mean ± the standard error of ...
Data are expressed as median and interquartile range; *p < 0.05, **p < 0.005, ***p < 0.001 as determined by one-way ANOVA with post-hoc Tukey’s multiple comparison test. Scale bar: 50 μm Full size image Fig. 6 Immunofluorescence staining of MPO⁺ and Ly-6G⁺ in ...
本试剂盒采用的是生物素双抗体夹心酶联免疫吸附法(ELISA)测定样品中小鼠白细胞介素6(IL-6)水平。向预先包被了小鼠白细胞介素6(IL-6)克隆抗体的酶标孔中加入小鼠白细胞介素6(IL-6),温育;温育后,加入生物素标记的抗IL-6抗体。再与链霉亲和素-HRP结合,形成免疫复合物,再经过温育和洗涤,去除未结合的酶,然后加入...
小鼠白细胞介素6IL6酶联免疫检测试剂盒使用说明书 AE90247Mu使用前仔细阅读本说明书。本酶联免疫试剂盒是基于双抗体夹心技术原理,来检测小鼠白细胞介素6IL6,只能用于研究用途,不得用于医学诊断。用途:用于小鼠血清血浆及相关液体样