Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms Il5, IL-5, Il 5, interleukin 5, interleukin-5Publications SpeciesSample TypeTitle mouse cell culture supernatants Int Immunopharmacol ...
Mouse IL-5 ELISA kit #EK0507 Review: Datasheet PDF add to favorites Catalog No.Package SizePriceQuantity EK05071x96T$449.00 Availability:The lead time is 1 month Total: ADD TO CART DatasheetImagesProtocol Description Product NameMouse IL-5 ELISA kit ...
4. Preparation of Biotin conjugated anti-Human IL-5 antibody (Kit Component 4) working solution: prepare no more than 2 hours before the experiment.a. Calculate the total volume of the working solution: 0.1 ml / well x quantity of wells. (Allow 0.1-0.2 ml more than the total volume)b....
Log-log graph paper or computer and software for ELISA data analysis Protocol Outline Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibo...
ELISA Detection1-3 or ELISPOT Detection4-6: The biotinylated JES6-5H4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JES6-1A12 antibody (Cat. Nos. 503701 & 503702) as capture antibody and recombinant mouse IL-2...
An Angiogenesis Starter Kit (Life Technology) was used to determine the formation of three-dimensional vessels according to the manufacturer’s protocol. Briefly, human umbilical vein endothelial cells (HUVEC) in supplemented Medium 200 (8000 cells/50 μl/well) were plated on Geltrex® Matrix-...
RNA immunoprecipitation (RIP) was performed using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, MA, USA) according to the manufacturer’s protocol. Chromatin immunoprecipitation assay The ChIP assay was performed using the ChIP Assay Kit (Millipore, MA, USA) following the ...
The experimental protocol was established, according to the ethical guide- lines of the Helsinki Declaration and was approved by the Human Ethics Committee of Jilin University, China. Writ- ten informed consent was obtained from individual partici- pants. Individual subjects with CHB were diagnosed, ...
By similar methods, rat hybridomas were derived from immunized rats, using a comparable immunization protocol and rat myelomas for the fusion as described for the mouse. Two rat hybridomas, 4A6 and 5D3, were identified that produced mAbs which bound to IL-5. Like mAbs 2B6, 2E3 and 2F2...
An effective amount can be the maximal dose or dosing protocol that avoids significant side effects or toxic effects. “Exogenous” refers to substances that are produced outside an organism, cell, or human body, depending on the context. “Endogenous” refers to substances that are produced ...