The sections were then placed into working nuclei isolation medium (NIM; 0.25 M sucrose, 25 mM potassium, 5 mM magnesium chloride, 10 mM TrisCl, 100 mM dithiothreitol, 1x protease inhibitor, RNase inhibitor) and triturated with a 1000 μL wide-bore pipette tip. After adding 0.1% Triton X...
Stat6 as an Inhibitor of Mastocytosis Another unexpected result of our studies is the observation that IL-4 induction of intestinal mucosal mastocytosis is actually enhanced in Stat6-deficient mice. This suggests both that IRS-2-mediated signaling is responsible for IL-4 induction of mucosal mast...
Tralokinumab is a potent inhibitor of IL-13 signaling through IL-13Rα1 in vitro and is efficacious in patients with moderate-to-severe AD, indicating that the high-affinity interaction between tralokinumab and IL-13 is sufficient to inhibit IL-13 binding to IL-13Rα1 and effectively ...
Being an important messenger in IL-4 signaling, we tested the effects of STAT3 inhibition in dependency of IL-4Rα expression. Therefore we used the specific inhibitor of STAT3-phosphorylationLLL12. Cells of BxPC-3, Capan-1, and PANC-1 wildtype as well as IL-4Rα knockdown cells 3-20 ...
The second band for IL-4Rα at 90 kDa was shown to be the non-glycosylated IL-4Rα by incubation of Capan-1-cells with Tunicamycin, an inhibitor of N-linked glycosylation (Figure S1A). In order to test the in vitro and in vivo influence of IL-4Rα on pancreatic cancer cells, we...