This adverse side effect could in principle be avoided by accurate IL2RG gene editing with a vector that does not contain a functional promoter or IL2RG gene. Here, we show that adeno-associated virus (AAV) gene editing vectors can insert a partial Il2rg cDNA at the endogenous Il2rg locus...
In addition, AKT activates EIF4E, a promoter of protein synthesis through mTOR and EIF4E had increased expression in activated cells. PI3K can also regulate cytotoxicity by activation of the RAC1, PAK1, MEK1/2 (MAP2K2) and ERK1/2 (MAPK1) pathways [9]. The expression of all these genes...
Mapping was performed with CellRanger (v5.0.1, with default parameters) on the mouse reference refdata-gex-mm10-2020-A to which the SV40 large TAG transgene was added (SV40 large TAG is expressed in beta cells under the rat insulin 1 promoter). A total of 17′105 barcodes and 32′286...