Effect of IL-4 on 11尾-HSD1-enzyme activity was measured in sorted and unsorted monocytes using Homogeneous Time-Resolved Fluorescence (HTRF) and M1/M2 polarization analysis was performed by flow cytometry. Our results indicate that CD14 cells are the major source of 11尾-HSD1 enzyme after IL...
(a) Schematic of the binary and ternary HTRF assays to assess the effect of tralokinumab on IL-13 association with IL-13Rα2 and IL-13Rα1 in the presence or absence of IL-4Rα. Three different experimental settings were utilized: setting I, where IL-13 and tralokinumab were preincu...
H2307T-H25F Size: 100 μg1 mg COA: Please contact us Price: Please contact us OnlineEmail Purchase+86-010-67809840 Description:Recombinant human IL4R protein with C-terminal Fc tag was purified by protein A affinity chromatography. The IL4R protein showed high activity in HTRF assay. ...
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通过体系中加入IL-17A&IL-17RA蛋白,随后加入相应的抗标签抗体连接的供体和受体检测液,若发生相互作用可以检测到高的HTRF信号值;当体系中加入抑制剂后,抑制剂会阻止IL-17A与IL-17RA之间的相互作用,无法结合使原本高的HTRF信号值降低,可根据HTRF信号值变化评价化合物对于IL-17A&IL-17RA结合活性的抑制能力。