(1)利用LV-EGFP慢病毒载体系统构建αIL17A:Fc融合蛋白的慢病毒表达质粒LV-αIL17A:Fc; 其中, αIL17A:Fc是IL-17A单链抗体与IgG4的重链Fc片段组成的融合蛋白, αIL17A:Fc编码基因的3’-末端先后连接IRES序列和EGFP基因, 形成αIL17A:Fc-I-EGFP序列,共同处于LV-EGFP慢病毒载体中CMV启动子的下游;(2)将LV-α...
Annexin V-EGFP Reagent BioVision-1004-1000 Annexin V-EGFP Reagent BioVision-1004-200 Annexin V Unlabeled Reagent BioVision-1005-100 Annexin V Unlabeled Reagent BioVision-1005-1000 Annexin V Unlabeled Reagent BioVision-1005-5000 10X Annexin V Binding Buffer BioVision-1006-100 Caspase-3 ...
To investigate the role of MCs in neutrophil TEM and TPM we crossed Mcpt5-Cre+-RDTA mice with LysM-EGFP-ki;α-SMA-RFPcherry-Tg animals, using the littermates Mcpt5-Cre--RDTA;LysM-EGFP-ki; α-SMA-RFPcherry-Tg (MCctrl) as controls (Fig. 2a). Applying confocal IVM to TNF-...
Interleukin (IL)-17a has been highly conserved during evolution of the vertebrate immune system and widely studied in contexts of infection and autoimmunity. Studies suggest that IL-17a promotes behavioral changes in experimental models of autism and agg
为了进一步阐明IL-17A通过作用于何种肠上皮细胞介导对于分泌型肠上皮细胞的调节,作者构建了分泌型肠上皮细胞特异 (Il17rafl/fl; Atoh1-cre) 和肠干细胞特异(Il17rafl/fl; Lgr5-EGFP-creERT2)的Il17ra敲除小鼠。作者发现,敲除ATOH1+细胞上的Il17ra不影响分泌型肠上皮细胞的数量,但在DSS炎症模型中却会加重结...
specifically Blocking inhibited TTGhF17-binatnodILP-1G7En2egFporoxdpu3cþtiocnellincodnivviedrusaiollny (Fig. 4d and Supplementary Fig. 4d,e). However, no significant reduction in the percentage of IL-17negFoxp3 þ cells when both TGF-b and PGE2 production were blocked demonstrates the ...