Importantly, IL-17RD mediates IL-17 signaling, as evaluated using a luciferase reporter driven by the native promoter of 24p3, an IL-17 target gene. In addition, an IL-17RD mutant lacking the intracellular domain dominant-negatively suppresses IL-17R-mediated IL-17 signaling. Moreover, IL-...
Here we use single-cell RNA sequencing, a molecular-clock reporter and genetic manipulations to show that innate IL-17-producing T cells—including γδ T cells, invariant natural killer T cells and mucosal-associated invariant T cells—are enriched for molecular-clock genes compared with their ...
Lymph-node (LN) stromal cell populations expand during the inflammation that accompanies T cell activation. Interleukin-17 (IL-17)-producing helper T cells (TH17 cells) promote inflammation through the induction of cytokines and chemokines in perip
2,3; however, the role of circadian rhythms in immune homeostasis is less well understood. Innate T cells are tissue-resident lymphocytes with key roles in tissue homeostasis4,5,6,7. Here we use single-cell RNA sequencing, a molecular-clock reporter and genetic manipulations to show that inna...
new T-cell lineage.26 The upregulation and function of these factors require signals from the cytokines transforming growth factor (TGF)-b and IL-6.27 IL-17A-producing Th17 cells are barely detectable in unmanipulated mice according to Hirota and colleagues,28 who used an IL-17A reporter system...
Recombinant human IL-17F (HZ-1116) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The ...
Although these data do not confirm that all IL-17+ CD8+ T cells express CCR6, data from an IL-17A-YFP reporter mouse have shown an increase in Ccr6 gene expression in YFP+CD8+ T cells indicating the expression of both CCR6 and IL-17A production is correlated to some extent [30]. ...
Recombinant human IL-17A (HZ-1113) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC...
Human IL-17 RA/IL-17 RC (Luc) HEK293 Reporter Cell 中文名: 中文别名: CBNumber: CB515555051 分子式: 分子量: 0 MOL File: Mol file 化学性质安全信息用途供应商1 Human IL-17 RA/IL-17 RC (Luc) HEK293 Reporter Cell化学性质 安全信息 ...
g effect of the miR-146a-3p on IL-17 was verified by the dual luciferase reporter assay. After isolation of the CD4+ T cells, the expression of miR ・l46a-3p in CD4+ T cells was promoted or inhibited by miR-146a-3p mimic or miR-...