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NotI 35 restriction endonucleases using polymerase chain reaction(PCR).Based on the wild type vector obtained, the mutant bases were introduced in both IL-10 and TLR4 primers using 3'UTR sequences as template, and 3'UTR sequences IL-10, TLR4 gene mutation were obtained by reverse transcription...
Feline IL-10 Primer Set 英文名称: Feline IL-10 Primer Set 总访问: 210 国产/进口: 进口 半年访问: 3 产地/品牌: kingfisher 产品类别: 细胞生物学试剂 规格: PS0202F-030 最后更新: 2024-12-5 货号: CAS 号: 参考报价: 询价 立即询价 电话咨询 ...
Human IL-10 qPCR Primer Pair: 基本信息 基因信息 种属: Human NCBI 参考序列号: NM_000572.2 产物大小: 101bp 产品信息 寡核苷酸类型: qPCR Primers 组分: 1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions). ...
The primer sequences for qRT-PCR are listed in Table S2. Results Serum from SLE patients with pleural thickening induced PMC remodeling Serum obtained from SLE patients was divided into two cohorts based on absence or presence of pleural thickening, namely SLE-control and SLE-pleural thickening (...
Primer(1 0u M) PCRReverse Primer(1 01.t M) ROXReference Dye II eDNA模板 dH20 Total 0.4Ul 0.4Ul 0.4|ll 2Ul 6.8II l 20Ul PCR扩增:应用ABI7500扩增仪 反应条件: 95℃0.5分钟 1个循环 I 95℃ 5秒钟 60℃34秒钟 40个循环 上 95℃ 15秒 ...
・中文论著摘要・NZW型IL.IORl信号转导机制及其对小鼠B淋巴细胞功能的影响在SLE中的作用刖吾系统性红斑狼疮(Systemiclupuserythematosus,SLE)是典型的全身性自身免疫性疾病,其发病及疾病严重程度与遗传因素有关。免疫学异常是本病发生的关键,涉及几乎所有单个核细胞及众多细胞因子。许多证据表明白细胞介素一10(Interl...
The following primers were utilized in sequences: Control human gene β-actin, 5′-GGA CTT CGA GCA AGA GAT GG-3′ (sense) and 5′-TGT GTT GGC GTA CAG GTC TTT G-3′ (antisense); Human tissue inhibitor of metalloproteinase 3 (TIMP3), 5′-CTG ACA GGT CGC GTC TAT GA-3′ (sense...
Expression levels were normalized to HPRT (DCt) and compared with littermate controls using the DDCt method.46 Primer sequences are: transforming growth factor-b: forward F, 50-CACAGTACAGCAAGGTCCTTGC-30; reverse R, 50-AGTAGACGATGGGCAGTGGCT-30; IL-12p35: F, 50-ATGACCC TGTGCCTTGGTAG-...
PrimePrimer5.0设计引 物:上游引物P1:5 ATAATTCTCGA~ ,CAGAGGA(X; CACCA 3,加入XhoI酶切位点(C~CGAG)。下游引物P2:5 GA(ACAAGCTTGAT( A HindIll酶切位点(AA(X2TI"),引物由上海博尚生物合成。扩增 片段长度为682bp。 1.2.2.基因组DNA提取 ...