TruSeq Stranded mRNA Library Prep kit (20020594, Illumina) was used to assess transcript abundance following the manufacturer’s instructions. In brief, poly(A) + RNA was purified from 1 µg of total RNA with RQS > 6, fragmented, and used for cDNA conversion, followed by 3′...
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Total RNA was extracted from the homogenized lung tissue and harvested cells by using TRIzol following manufacturer’s instructions (Thermo Fisher). The reverse transcription was conducted with the Advantage RT-for-PCR Kit (Takara), and cDNA products were used for the quantitative real-time PCR (...
All Special IssuesFeature Annual Reviews in Molecular Sciences 2019Single Molecules ANDOR All fieldsTitleAbstractKeywordsAuthorsAffiliationsDoiFull TextReferences Open AccessArticle by Takehisa Nakanishi 1, Shohei Iida 1, Masako Ichishi 2, Makoto Kondo ...
Purified CD4 T cells were stained with anti-CD25 PE (2A3 clone) at a concentration of 5 μL/1 × 106 cells for 30 minutes on ice, washed, and subsequently incubated with anti-PE MicroBeads (Miltenyi Biotec) according to the manufacturer's instructions. The CD4+CD25hi fraction was ...
安纳白介素: 一种IL1R1拮抗剂药物,由Amgen, Inc. (Amgen, Inc.)公司最早进行研发,目前全球最高研发状态为批准上市,作用机制: IL1R1拮抗剂(白细胞介素-1受体拮抗剂),治疗领域: 免疫系统疾病,感染,遗传病与畸形,在研适应症: 白细胞介素-1受体拮抗剂缺乏,Muckel-阱综合征,
IL-23R抑制剂 在研机构 Janssen Pharmaceutica NV[+4] 原研机构 Protagonist Therapeutics, Inc. 在研适应症 红皮病性银屑病[+4] 非在研适应症- 最高研发阶段临床3期 首次获批国家/地区- 首次获批日期1800-01-20 GEN-1 靶点 NCT06217666/Not yet recruiting临床1期IIT ...
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Total RNA was extracted from the homogenized lung tissue and harvested cells by using TRIzol following manufacturer’s instructions (Thermo Fisher). The reverse transcription was conducted with the Advantage RT-for-PCR Kit (Takara), and cDNA products were used for the quantitative real-time PCR (...
cells were washed and GSH/GSSG Glo™ Assay (Promega, V6611) was used or ROS levels were determined by incubation with 1 µM chloromethyl derivative of 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA, Thermo Fisher Scientific, C6827) according to the manufacturer’s instructions. ...