Infiltration, accumulation, and survival of chimeric antigen receptor T (CAR-T) cells in solid tumors is crucial for tumor clearance. We engineered CAR-T cells to express interleukin (IL)-7 and CCL19 (7 × 19 CAR-T cells), as these factors are essential for the maintenance of T-cell zo...
并且发现7x19 CAR-T细胞与抗CD3抗体或表达hCD20的P815肥大细胞瘤(P815-hCD20)共培养时产生了IL-7和CCL19,成功使CAR-T细胞在自身没有受到影响的情况下分泌出IL-7和CCL19。 由于已知 IL-7 可增强 T 细胞的增殖和存活随后该团队针对IL...
Li L, Zhao M, Wu G, Ouyang G, Liu M, Zhang J, Gao J, Qian W. Safety and feasibility of anti-CD19 CAR T cells expressing inducible IL-7 and CCL19 in patients with relapsed or refractory large B-cell lymp...
Moreover, this response is associated with an increase of DC with a capacity of cross presentation to T cells, which is a crucial machinery for the epitope spreading. Reference Adachi K, Kano Y, Nagai T, Okuyama N, Sakoda Y, Tamada K. IL-7 and CCL19 expression in CAR-T cells ...
3、2022年《MOLECULAR CANCER THERAPEUTICS》上发表了文章《Enhanced Antitumor Responses of Tumor Antigen-Specific TCR T Cells Genetically Engineered to Produce IL7 and CCL19》,研究证实IL-7和CCL19在免疫细胞治疗方面具有积极作用,不仅仅体现在CAR-T细胞治疗领域[5],在TCR-T细胞治疗领域[6],也依然有效。我们...
为证实该CAR-T的优异的性能表现是因为IL-7和CCL19的影响,该团队使用了抗CD127抗体来抑制细胞上IL-7的受体,从而降低IL-7的作用。使用抗CCR-19的抗体来抑制CCL19的受体降低CCL19的作用。7 × 19 CAR-T细胞的强增殖能力被抗CD127抗体削弱了,但使用抗CCR7抗体则无影响,表明IL-7对于7 × 19 CAR-T细胞中细胞增...
3、2022年《MOLECULAR CANCER THERAPEUTICS》上发表了文章《Enhanced Antitumor Responses of Tumor Antigen-Specific TCR T Cells Genetically Engineered to Produce IL7 and CCL19》,研究证实IL-7和CCL19在免疫细胞治疗方面具有积极作用,不仅仅体现在CAR-T细胞治疗领域[5],在TCR-T细胞治疗领域[6],也依然有效。我们...
IL-7 and CCL19 expression in CAR-T cells improves immune cell infiltration and CAR-T cell survival in the tumor Nat. Biotechnol., 36 (2018), pp. 346-351 CrossrefView in ScopusGoogle Scholar [21] B.D. Choi, X. Yu, A.P. Castano, A.A. Bouffard, A. Schmidts, R.C. Larson, S...
Chimeric antigen receptor (CAR) T cell therapeutic responses are hampered by limited T cell trafficking, persistence, and durable anti-tumor activity in solid tumors. However, these challenges can be largely overcome by relatively unconstrained synthetic
Th2型细胞能分泌IL-4、IL-5等细胞因子,与B淋巴细胞增殖、分化、成熟有关,能促进抗体生成,增强抗体介导的体液免疫应答,还分泌IL-10、IL-13以及CCL-7等