4. Cells should be passaged when a 70-80% confluency is reached. Do not let the cell grow to 100% confluency. DETECTION OF IL-17C ACTIVITY We recommend to use test medium one passage prior to the assay. Day 1: 1. Prepare HEK-Blue™ IL-17C cell suspension: gently rinse cells...
HEK-Blue™ IL-17C cells allow the colorimetric detection of bioactive human and mouse interleukin-17C (IL-17C) and IL-17E (IL-25). They can be used for the screening of anti-IL-17 antibodies or for IL-17 cell-based potency assay.
(A) Overall structure of the Fab/HAP/IL- 17A/compound 1 complex. Component polypeptides appear as ribbons, with two HAP molecules in blue and red, respectively, and the two chains of IL-17A in ice blue and gold, respectively. Atoms of compound 1 appear as spheres, with carbons in green...
IL-17A is secreted from activated Th17 cells, and several innate immune T cell types including macrophages, neutrophils, natural killer cells, and dendritic cells2. IL-17A signals through a specific cell surface receptor com- plex which consists of IL-17RA and IL-17RC3. IL-17A's downstream ...
Cell surface staining was performed on Human IL-23 R/IL-12 R beta 1 (Luc) HEK293 Reporter Cell or negative control cell using PE-labeled anti-IL-23 R antibody and APC-labeled anti-IL-12 R beta 1 antibody.参考文献 1.Krueger, James G., et al. "IL-23 past, present, and future: ...
In HEK293T cells, overexpressed IL-17B can indeed interact with both IL-17RA and IL-17RB (Figure S2I). However, immunoprecipitation showed that IL-17B only interacted with IL-17RB in injured nerves (Figure 3H), suggesting a potential activation of IL-17B/IL-17RB autocrine signaling in ...
Transfection assay in HEK293-hTLR5 cells enables IL-8 secretion in response to C. sakazakii flagella and flagellin.Ariadnna, CruzCórdovaLuz, M. RochaRamírezSara, A. OchoaBertha, GónzalezPedrajoNorma, EspinosaCarlos, EslavaUlises, HernándezChiñas...
HEK-Blue IL22 cells were grown on a surface treated with Poly-D-Lysine (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) to ensure uniform attachment without cell clamping which was observed on an untreated surface. Inhibition assay was carried out in DMEM with 2 mM L-glutamine, 5.4 g/...
However, recent studies found that co-expression of IL-17F and IL-17A in HEK293 cells results in the formation of biologically active IL-17F/IL-17A heterodimers, in addition to the IL-17F homodimers and IL-17A homodimers. Activated human CD4+ T cells were found to produce the IL-17A...
amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of intere...