miltenyi美天旎human IL-15蛋白,人白介15细胞生长因子IL-15 stands for interleukin 15. Human IL-15 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays. 详细介绍 miltenyi美天旎human IL-15蛋白,人白介15细胞生长因子,miltenyi美天旎human IL-15蛋白,...
预测的最佳IL7/IL-15剂量显示细胞数量略有增加,分别是17倍(Stemcell)和22倍(Miltenyi)。两种激活剂的单次IL-2剂量分别是22倍(Stemcell)和19倍(Miltenyi)。 图5 利用T细胞耗竭标志物进行表型鉴定。细胞质量的评估是基于各自的同型对照的门控的CD4+和CD8+T细胞亚群。在培养结束时,对细胞在补充细胞因子前后进行分析...
图2B和2C展示质粒IL-15Ra表达了适当大小的蛋白质(约30kDa),分别由用R&D和KKl. 23单克隆抗人IL-15R α抗体进行的放射性活体外翻译来检测。图2D展示单克隆KKl. 23抗体(IgGl同种型)不结合非转染的HeLa细胞(20χ)。图2Ε展示由小鼠IgGl同种型对照染色的pTRACER-IL-15Ra转染细胞(绿色)(20x)。图2F和2G分别...
将细胞洗涤一次并与经抗PE抗体共轭的磁珠(MiltenyiBiotec)于4C培养15分钟。将细胞载入磁性管柱(于磁场中),并以洗涤缓冲液(含有0.5%BSA的PBS)去除未结合的细胞。将管柱从磁场中移走后,以含有10%FBS的IMDM冲提与管柱结合的细胞。基于在制造/分泌过程的期间细胞表面上的可溶性融合蛋白的短暂呈现,此程序使融合蛋白...
产品名称: Human IL-15, premium gr. (100 μg) .0 订购此产品 供应商: miltenyi 规格: *** 目录价: 8014.5 库存状态: CAS编号: 应用范围: 种属来源: 相关信息: 保存条件: 说明书地址: 点击查看详细 打印此页 关闭此页 上一个:Human IL-15, premium gr. (25 μg)[130-095-764/***...
a Biochemical byMiltenyi Biotec (17144002)Ficoll-Paque PLUS a Biochemical byCytiva (276855)Dimethyl sulfoxide a Biochemical byMilliporeSigma (560098)BD Pharmingen™ Human FoxP3 Buffer Set a Biochemical byBD Biosciences (B6542)Brefeldin A a Biochemical byMilliporeSigma ...
Spleen and LN cells from B6 mice or where indicated from IL-15 Rako mice were enriched for CD4 þ T lymphocytes by negative selection using the CD4 T-cell isolation kit and autoMACS-Pro separator (Miltenyi Biotec, Germany). For other transfers, spleen and LN cells from Foxp3-mRFP or ...
(Banc de Sang i Teixits, Catalonia). Human T cells were isolated from buffy coats by density-gradient centrifugation using Histopaque-1077 (Sigma-Aldrich, St Louis, MO, USA) followed by negative selection of T cells using a Pan T Cell Isolation Kit (Miltenyi Biotech, Bergisch Gladbach, ...
miltenyi美天旎Human IL-2 IS细胞因子品牌:Miltenyi(美天旎)货号:130-097-748CAS号:详见说明书供应商:Miltenyi(美天旎)保质期:详见说明书保存条件:详见说明书规格:EA 详细介绍 miltenyi美天旎Human IL-2 IS细胞因子,miltenyi美天旎Human IL-2 IS细胞因子, IL-2 IS stands for interleukin 2 “Improved Sequen...
The left lung lobe was excised post BAL, crudely dissociated using the GentleMACS tissue dissociator (Miltenyi Bio- tech, Surrey, UK) and digested upon incubation at 37 1C in buffer containing 1 mg ml À 1 collagenase Type XI and 80 units ml À 1 Bovine Pancreatic DNase Type IV (...