RNA was isolated from single cell suspensions using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions. RNA integrity was confirmed using Agilent’s 2200 Tapestation. Samples were processed using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech Laboratories, Inc.). Brief...
A Correction to this article was published on 03 December 2024 This article has been updatedAbstract IKK signalling is essential for survival of thymocytes by repressing RIPK1 induced cell death rather than its canonical function of activating NF-κB. The role of IKK signalling in activated T cell...
Article Open access 21 July 2024 References Hoffman EP, Monaco AP, Feener CC, Kunkel LM . Conservation of the Duchenne muscular dystrophy gene in mice and humans. Science 1987; 238: 347–350. Article CAS PubMed Google Scholar Lapidos KA, Kakkar R, McNally EM . The dystrophin glycoprotein...
Layzell1, Alessandro Barbarulo1, Geert van Loo 2, Rudi Beyaert 2 and Benedict Seddon 1 ✉ © The Author(s) 2024, corrected publication 2024 IKK signalling is essential for survival of thymocytes by repressing RIPK1 induced cell death rather than its canonical function of activating NF-κB...
Immunoreactive bands were visualized by enhanced chemiluminescence using the Western Lightning Chemiluminescence Reagent Plus (Perkin Elmer Life Sciences) and a CCD-camera LAS400 mini apparatus (GE Healthcare). Statistical analysis Associations between IKKε expression status (positive vs. negative) and ...