The staining score of HIF-1α, VEGF and TGF-β1 in peritoneum by IHC (X̅±s).Juan, Li
An H-score of more than 1 is positive. PgR was quantified by the percentage of cells staining positive, and this was divided by 10 to obtain a variable between 0 and 10 (PgR10). HER2 assessment was given as either a positive (IHC3 þ or IHC2 þ and CISH or FISH positive) or ...
Traditional indications for HER2-directed therapy in breast cancer patients have been based on immunohistochemical (IHC) staining on tumor tissue. Per the National Comprehensive Cancer Network (NCCN) guidelines, these therapies are indicated for patients that score 2+ or 3+ on IHC with evidence of ...
AI对不同异质性类型的判读准确性均有不同程度的提高,特别是对分散型(图3D)。 图3. 两轮读片的异质性分析:(A)HER2 0(no staining)、HER2超低表达、HER2 1+病例的异质性分布;(B)两轮读片对同质性和异质性病例的诊断准确性;(C)HER2超低表达、HER2 1+病例中的3种异质性类型分布;(D)两轮读片对3种异质性...
使用误差矩阵(Confusion Matrix)、准确性(Accuracy)、精准性(Precision)、召回率(Recall)、F1分数(F1-Score,精确性和查全率的调和平均数)和Cohen’s Kappa系数来评价诊断性能;使用组内相关系数(ICC)评价一致性;使用Kruskal-Wallis和Wilcoxon ...
Ki-67 scores were recorded as the percentage of positively staining cancer cells using the MIB1 antibody, in five representative high-power fields (Zabaglo et al, 2010). The IHC4+C score was calculated using the addition of two algorithms: (i) The IHC4 score using an adjustment for Ki67...
Staining protocols for human pancreatic islets. The final component described in part 2 is the provision of the stained slides as digitized whole slide images. The digitized slides are organized by ... ML Campbell-Thompson,T Heiple,E Montgomery,... - 《Journal of Visualized Experiments Jove》 ...
Results: Cases were assessed as IHC score 0 (no staining; n = 69), 1+ (faint cytoplasmic staining, n = 21), 2+ (moderate, smooth cytoplasmic staining; n = 3), or 3+ (intense, granular cytoplasmic staining in ≥10% of tumor cells; n = 8). All IHC 3+ cases were FISH+, ...
(GEJ) tissue specimens by light microscopy. This assay is used with OptiView DAB IHC Detection Kit for staining on a BenchMark ULTRA instrument. The assay is FDA-approved and is indicated as an aid in identifying patients with gastric or GEJ adenocarcinoma who may be eligible for treatment ...
Score Definition 0 No immunostaining 1+ Weak immunostaining, less than 30% of tumor cells 2+ Complete membranous staining, either uniform or weak in at least 10% of cells 3+ Uniform intense membranous staining in at least 30% of cells IHC validation One important new requirement of the ASCO...