The following immunohistochemistry (IHC) protocol has been developed and optimized by R&D Systems IHC/ICC laboratory for fluorescent immunohistochemistry staining experiments using paraffin-embedded tissue samp
Immunohistochemistry On Paraffin Sections Please note: All steps in the following protocol are carried out at room temperature unless stated otherwise. Deparaffinizing and rehydration Immerse slides in xylene for 10 minutes. Repeat this step again in fresh xylene for a further 10 minutes. ...
Clear in xylene 2 times for 5 minutes. Coverslip with mounting medium. Tips for Immunohistochemistry Technical Tips View Technical Tip Heat-induced Epitope Retrieval Protocol Protocols View Protocol Staining Paraffin Sections by PAP Procedure Protocols ...
Step-by-step protocol and tips for immunohistochemistry on paraffin-embedded sections. Download PDF version Preparing Fixed Tissues for Paraffin-Embedded Sectioning Here we describe the procedure for preparing formalin-fixed paraffin-embedded tissue (FFPE) samples. ...
Jump to: Immunohistochemistry Overview IHC preparation IHC On Paraffin Sections IHC on Frozen Tissue IHC Antigen Retrieval IHC Blocking Selecting Antibodies For IHC IHC Sample Visualization IHC Troubleshooting IHC ppt Immunohistochemistry Video Protocol
Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections 查看更多about Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections ...
Primary Antibodies for IHC-Paraffin-Embedded Sections View all IHC-validated Primary Antibodies Find Your IHC Staining Protocol: Chromogenic Staining of FFPE Tissue Protocol Chromogenic Staining of Frozen Tissue Protocol Fluorescent Staining of FFPE Tissue Protocol Fluorescent Staining of Frozen Tissue Protocol...
Clear paraffin so that tissue is exposed and re-hydrate. Remove wax by soaking sample in xylene (or another detergent) at least for 60 min. Place staining rack sequentially in different concentrations of alcohol including; 100% 2x, 95%, 85%, 75%, 60%. Leave staining rack in each alcoho...
Tips to help you select the right antibody for your IHC staining.Jump to: Immunohistochemistry Overview IHC preparation IHC On Paraffin Sections IHC on Frozen Tissue IHC Antigen Retrieval IHC Blocking Selecting Antibodies For IHC IHC Sample Visualization IHC Troubleshooting...
Use coated slides. Place slides in covered dish with a small amount of 16% formaldehyde on the bottom to fix the tissues to the slides. Be gentle when using an antigen retrieval method and avoid heavy agitation of the slides. Back to IHC-Paraffin Protocol...