流式免疫分析显示异常细胞主要表型为CD5-CD10-,CD103+CD11C,CD25-CD123-,与毛细胞白血病变异型的特征相符。分子生物学检测发现IGH FR1-JH、IGH FR2-JH、IGH DH-JH、IGK Vk-Jk、IGK Vk-Kde+intron-Kde基因重排,提示B细胞淋巴瘤可能。淋巴结穿刺活检与骨髓活检...
本研究主要检测B-ALL患儿骨髓样本中IGH基因的可变区(V)、多变区(D)、连接区(J)及IGK的V、J区,包括:IGH VH-FR2、IGH VH-FR3、IGH DH-JH、IGK Vk-Jk/Kde(优势克隆序列指占比≥5%的序列,即克隆序列的有效测序读数与样本中有...
复查igh基因，显示dh-jh阳性，诊断igh基因重排。这说明就有微小残留病灶吗？伯基特淋巴瘤结疗11月复查 ...
复查igh基因，显示dh-jh阳性，诊断igh基因重排。这说明就有微小残留病灶吗？伯基特淋巴瘤结疗11月复查 ...
The DH to JH rearrangement step occurs in both Igh alleles7. This event has recently been proposed to mark the DJH junction for secondary rearrangement, that is, the VH to DJH stage8, and once a productive secondary rearrangement takes place in one of the alleles of the pro-B cell, expre...
结论采用PCR片段分析法可有效地区分单克隆、多克隆、寡克隆,从而排除多克隆产物的干扰,降低假阳性率,对临床鉴别诊断具有重要的意义。IGH VH-JH+IGH DH-JH+IGK联合检测可大大提高检出率,尤其在IGH VH-JH重排阴性的MM患者IGH DH-JH重排的补充尤为... 艾晓非,张岩,徐宁,... - 全国血液肿瘤学术大会暨全国淋巴肿...
The most numerous genes consist of unjoined VH-D1-D2-JH segments that correspond to the most abundant Hydrolagus spleen (cDNA) transcripts which apparently arise from a diversified gene family. In the second cluster type, VH, DH, and JH segments are germline-joined, whereas the CH exons ...
很早就出现工gH基因重排,重排时DH与JH首先重排,即一个DH片 段与一个JH片段结合,去掉DH与JH之间的核昔酸序列,然后是一 个VH与已重排的DH-JH结合去掉VH与DH之间的核普酸序列,最后 再与恒定区基因片段 (CH) 重排,形成完整的工gH基因。重排过 程中V叮基因片段之间的结合是随机的且随机结合的片段之间可 ...
Interestingly, two of those clones were essentially depleted of the VH–DH intergenic fragment (Fig. 5a), showing that these populations are mostly composed of cells that also have the silenced allele in the VHDJH configuration. This observation suggests that the extreme bias in this subset of ...
2,Dixon MF,Genta RM,Yardley JH,et al.Classification and grading of gastritis.Am J Sur Pathol,1996,20:1161-1170. 3,Isaacson PG,Spencer J,Wright DH.Classifying primary gut lymphomas.Lancet,1989 19、,ii:1149. 4,Sukpanichnant S,Vnencakjones CL,Mc Curley TL,et al.Determination of B-cell ...