Finally, 50 µl PB containing 50 U of immunoglobulin degrading enzyme (IgdE; branded FabALACTICA, Genovis AB, Lund, Sweden) was added, which selectively cleaves only IgG1s in their hinge region, before incubation on a thermal shaker at 37 °C for at least 16 h. After overnight...
Peptide corresponding to the hinge region of Human IgG1 偶联物 Biotin 形式 Liquid 浓度 1 mg/mL 纯化类型 Protein A 保存液 PBS with 1% BSA, 50% glycerol 内含物 0.09% sodium azide 保存条件 -20°C 运输条件 Ambient (domestic); Wet ice (international) ...
More detailed analysis of the chemical changes was investigated using model linear and cyclic peptides (with the sequence of SCDKTHTC) derived from the upper hinge region of the mAb. Cu(2+) mediated fragmentation was determined to be predominantly via a hydrolytic pathway in solution. The sites...
Hinge mutagenesis studies revealed that disrupting the core hinge (CPPCP; Fig. 2) leads to reduced IgG1 binding to FcγRIIIA (52). Sequence differences in the IgG subclass heavy chains may also be relevant, with IgG1 and IgG3 possessing Leu234 and Leu235 in the lower hinge region, ...
34 It is considered that the hinge region acts as a spacer, effecting segmental flexibility and allowing the Fab arms to move in relation to the Fc.35 A study showed that changes in the amino acid sequences could affect the rigidity of the hinge, which in turn affected the effector ...
pFUSE-hIgG1-Fc are cloning plasmids for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the IgG1 heavy chain and the hinge region. pFUSE-hIgG1-Fc plasmids are now available with or without the IL2 signal sequence.
pFUSE-hIgG1e13-Fc2 Plasmid containing a human engineered IgG1 Fc region Catalog # pfc2-hg1e13 For research use only Version 20K04-MM ProduCt information Content: - 20 µg of pfuse-higG1e13-fc2 (il2 ss) plasmid provided as lyophilized DNA - 1 ml of Zeocin™ (100 mg/ml) storage ...
A unique BglII site was introduced at the 5' end of the inserted Fc encoding sequence, nucleotides 1 through 6 (AGA TCT) in SEQ ID NO 3. The Fc polypeptide encoded by the DNA extends from the N-terminal hinge region to the native C-terminus, i.e., is an essentially full-length ...
产品参数 载体介绍 pFUSE-hIgG1-Fc1 is a cloning plasmid for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the human IgG1 heavy chain and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, ...
[2]. Activated PD-1 enables cancer cells to evade the immune system. Pembrolizumab binds and inhibits the PD-1 receptor, thereby resulting in the activation of T cells. This therapeutic mAb contains an engineered hinge region mutation (S228P) designed to prevent the exchange of IgG4 molecules...