最后，研究人员为研究IGF1刺激是否能逆转中年小鼠LT-HSC的表型，发现IGF1直接刺激能引起IGF1R及AKT磷酸化，降低体外骨髓分化，逆转HSC老化的多种分子表型，而不影响HSC本身的自我更新。进一步发现IGF1刺激能影响中年LT-HSCs的染色质可接近性，并在转录水平增加多个代谢通路特征基因。人在成年后，体内系统生成的IGF1水平...
由于IGF1信号调节淋巴祖细胞的B淋巴细胞生成,接下来直接检测了造血干/祖细胞(HSPCs),进一步发现骨髓微环境中IGF1的降低并不能充分支持淋巴细胞的产生,而有利于HSPC的髓系造血。 为了了解IGF1是否能使中年LTHSC表型恢复活力,研究者在体外培养系统中加入IGF1,显示可持续地减少偏髓性造血,增加淋巴潜能,而不影响HSC的自我...
This niche reorganization fosters homing of the transplanted hematopoietic cells to the host marrow space and engraftment of long-term-HSC. Inhibition of insulin-like growth factor (IGF)-1-receptor tyrosine kinase signaling abrogates endosteal osteoblast proliferation and donor HSC engraftment, suggesting ...
本课题主要研究IGF-1在人NK细胞发育及功能中的调控作用及其机制,进一步完善NK细胞的基础生物学理论,并为NK细胞的免疫治疗提供实验基础.本课题首先分离新生儿脐带血CD34+造血干细胞(HSC),加入干细胞因子(SCF),Fit3配体(Flt3L)和白介素-15(IL-15)进行长期培养(28天),进行体外NK细胞的分化及扩增,通过在该体系中加...
we clearly show that IGF-1 stimulates the perforin gene and subsequent protein expression in HSC-derived NK cells and CD56brightdNK cells. Moreover, IGF-1 induced a steep rise in the cytotoxic effector function of dNK cells. We further demonstrate that the STAT3 pathway is involved in the enh...
活化的肝星状细胞(HSC)能分泌多种细胞因子如转化生长因子-β1(TGF-β1),胰岛素样生长因子-1(IGF-1)等[1],其中TGF-β1是肝纤维化发生发展中最关键的细胞因子.目前对肝... 徐叶进,陈凌,郭玉香,... - 《中华临床感染病杂志》 被引量: 4发表: 2015年 ...
Positive WB detected in HEK-293 cells, HepG2 cells, HeLa cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells, mouse placenta tissue Positive IP detected in HeLa cells Positive IHC detected in human pancreas cancer tissue, human cervical squamous cancer tissue, human colon cancer tissueNote: sug...
HSC70 served as a loading control. c Impaired cell proliferation upon EHD1 knockdown. The indicated shNTC and shEHD1 TC71 or A673 cell lines pre-treated with Dox for 48 h were plated in 96-well plates and cell proliferation assessed at the indicated time points using the Cell-Titer-Glo ...
Objective To study the effect of vitamin D on β cell function, hsC-RP, and the lipoprotein metabolism in T2DM patients through an observation on the chang... 陈宽林,华云峰,卓铁军 - 《中国糖尿病杂志》 被引量: 0发表: 2013年 振源胶囊辅助治疗2型糖尿病对患者空腹C肽水平及胰岛素抵抗的影响 (...
Novel role of C terminus of Hsc70-interacting protein (CHIP) ubiquitin ligase on inhibiting cardiac apoptosis and dysfunction via regulating ERK5-mediated degradation of inducible cAMP early repressor. FASEB J 2010; 24: 4917–4928. 26. Kim SA, Yoon JH, Kim DK, Kim SG, Ahn SG. CHIP ...