3T3-L1細胞胰島素樣生長因子-1地塞米松胰島素脂肪水孔蛋白 3T3-L1 adipocytesIGF-1insulindexamethasoneaquaporin adiposeObjective: To investigated the effect of hormone such as insulin, IGF-1 and dexamethasone on expression of AQPap in 3T3-L1 adipocytes, in order to know the role of AQPap in obesity ...
5.Magun R, Boone DL, Tsang BK, et al. The effects of adipocyte differentiation on the capacity of 3T3-L1 cells to undergo apoptosis in response to growth factor deprivation. Int J Obelat Metab Disord, 1998, 22: 567-571. 6.Coppak SW. Pro-inflammatory cytokines and adipocyte tissue. Pro...
生物活性: ED50由Balb/c 3T3细胞对胸苷摄取的剂量依赖性刺激所确定,ED50≤1 ng/ml,对应的比活性≥1 x 106units/mg。
Insulin regulation of sterol regulatory element-binding protein-1 expression in L-6 muscle cells and 3T3 L1 adipocytes J Biol Chem, 279 (2004), pp. 34380-34387 View PDFView articleView in ScopusGoogle Scholar Pfaffl et al., 2002 M.W. Pfaffl, G.W. Horgan, L. Dempfle Relative expression...
, cells and mouse embryo fibroblasts; however, insulin/IGF-1 did not promote degradation of IRS-1 in 3T3-L1 preadipocytes or mouse embryo fibroblasts. ... L Rui,TL Fisher,J Thomas,... - 《Journal of Biological Chemistry》 被引量: 536发表: 2001年 Differential signalling potential of insuli...
The ED50 of recombinant human Long R3 IGF-I, calculated by the dose-dependant proliferation of murine BALBC 3T3 cells, is less than 1.0ng/ml, corresponding to a specific activity of 1.0×106IU/mg.Endotoxin: ﹤ 1.0 EU/mg as determined by the LAL method. Product specification: ...
引入EcoR I、Hind III酶切位点,在天然的IGF-1前面添加一个Asn,形成一个羟胺的特异裂解部位,降低了蛋白切割成本,减少额外氨基酸序列对蛋白复性的影响,保持蛋白在天然条件下的状态;采用pET-32a作表达载体在大肠杆菌Rosetta中表达,蛋白含量可达菌体可溶蛋白的50%以上,通过MTT法检测,能提高NIH3T3细胞增殖速度。 法律状态...
a.ii)测试方案第一天收获正以指数生长的NIH3T3/IGFR细胞,并以100μl的体积按照1.2×104个细胞/孔在完全生长培养基中接种至平底的96孔组织培养等级板(Costar 3525)中。 第二天采用多道移液器小心将生长培养基从每个孔中取出。用200μlHBSS仔细将孔漂洗3次。将100μl饥饿培养基加入到每个孔中,将板再培养24小时...
(DL-Dithiothreitol,DTT),25℃,摇床酶切16 h,可实现1 U凝血酶切割100μg融合蛋白;通过酶切前后两次亲和层析可获得纯度大于90%的目的蛋白;利用3T3细胞增殖法建立了IGF-1体外活性评价体系,该体系检测条件为铺板细胞密度1×104/mL,初始,铺板及维持培养基血清浓度分别为4%,2%与1%,IGF-1样品初始浓度1000 ng/mL,3...
3T3-L1细胞 293细胞 293T细胞 293T/17细胞 293FT细胞;IGF-1 ELISA试剂盒, 玉博生物商城|www.ybiotechmall.com 产品留言 标题 联系人 联系电话 内容 验证码 点击换一张 注:1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!2.如有必要,请您留下您的详细联系方式! 相关产品 生化试剂 PCNA...