ABC Clonal大鼠胰岛素样生长因子1 ELISA试剂盒（IGF1）RK03737 V2.0说明 Rat Insulin Like Growth Factor 1ELISA Kit(IGF1)Catalog NO.:RK03737 version:2.0 *使用产品前，请务必阅读产品包装内说明书 1 www.abclonal.com
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(GTX103042, Gene Tex), rabbit anti-Cyclin D1 (2978 S, CST), rabbit anti-Cyclin E1 (340298, ZEN BIO), mouse anti-PCNA (200947, ZEN BIO), rabbit anti-MyoD (252249, ZEN BIO), mouse anti-MyHC (MAB4470, R & D Systems), rabbit anti-Fbx32 (A3193, Abclonal), rabbit anti-MyoG (...
Deparaffinized tissue sections were blocked and incubated with anti-IGF-1 (1:100, Servicebio, China), anti-COL1 (1:100, Servicebio, China) and anti-TNC (1:200, ABclonal, China) overnight. Specimens were washed three times in PBS before being incubated with secondary antibodies conjugated ...
The membranes were then incubated with the following primary antibodies overnight at 4°C: Anti-IGF1 (1:1,000; cat. no. A12305; ABclonal Biotech Co., Ltd.), p-c-Raf (1:1,000; product code ab150365; Abcam), anti-c-Raf (1:1,000; product no. 53745; Cell Signaling Technology, ...
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Immunohistochemistry for VEGF and Kiss-1 was done using a primary polyclonal rabbit anti-VEGF-A (ABclonal, Cat. No. # A0280) and a primary rabbit polyclonal anti-kisspeptin antibody (Bioss, Cat. No. # bs-0749R) and their related secondary antibodies. Antigens were retrieved via boiling ...
Small interfering RNAs (siRNAs) targeting FTO (siFTO) and scrambled siRNA (siNC) were obtained from RIBOBIO (Guangzhou, China). Glioma cell lines were transfected with plasmids using HighGene plus Transfection reagent (ABclonal Technology, Wuhan, China), following the manufacturer’s instructions. For...
The sections were then blocked in 5% normal sheep serum with 0.1% Triton for 2 h and incubated with anti-FTO (Proteintech, 1:500), anti-CDH12 (Abclonal, 1:200), anti-IGF2BP2 (Proteintech, 1:500) and anti-m6A (Abclonal, 1:200) at room temperature for 2 h, followed by ...
Bound secondary antibody (anti-rabbit, 1:5,000, SA00001-2, Proteintech, or anti-mouse, 1:5,000, AS003, ABclonal, China) was detected using the enhanced chemiluminescence (ECL) reagent (170506, Bio-Rad, Hercules, USA). 2.5 RNA extraction and sequencing The DNA was removed from total RNA...