Linearized DNA of the Ig expression vector plasmid was introduced into NSO or Sp 2/0 cells by electroporation using the Gene Pulsar instrument (Bio-Rad, Richmond, Calif.) at 200 V and 960 μFd. Transfected cells were selected in Iscove's modified Dulbecco's medium (GIBCO) containing 2% FCS...
As of January 2014, the state has roughly 1080 zip codes, which are listed below with the respective city, town, village or location name as well as the area code. The capital is Raleigh (2012 population: 423,179) and the largest city is Charlotte (2012 population: 775,202). North Car...
As of January 2014 Virginia has roughly 1216 zip codes, which are listed below with the respective city, town, village or location name as well as the area code. The capital is Richmond (2012 population: 210,309) and the largest city is Virginia Beach (2010 population: 437,994). Virginia...
As of January 2014, Florida has roughly 1469 zip codes, which are listed below with the respective city, town, village or location name as well as the area code. The state's capital is Tallahassee (2012 population: 181,376) and its largest city is Jacksonville (2012 population: 836,507)...