Linearized DNA of the Ig expression vector plasmid was introduced into NSO or Sp 2/0 cells by electroporation using the Gene Pulsar instrument (Bio-Rad, Richmond, Calif.) at 200 V and 960 μFd. Transfected cells were selected in Iscove's modified Dulbecco's medium (GIBCO) containing 2% FCS...
At 163,696 square miles, California is the 3rd largest state in America. Administratively California is divided into 482 cities and towns and 58 counties. The state's population is 38,332,521, according to the 2013 United States Census, making it the 1st most populous state in America. As ...
As of January 2014 Virginia has roughly 1216 zip codes, which are listed below with the respective city, town, village or location name as well as the area code. The capital is Richmond (2012 population: 210,309) and the largest city is Virginia Beach (2010 population: 437,994). Virginia...
At 53,819 square miles, North Carolina is the 28th largest state in America. Administratively North Carolina is divided into 552 cities and towns and 100 counties. The state population in 2013 was 9,848,060 making it the 10th most populous state in America. As of January 2014, the state ...
At 65,755 square miles, Florida is the 22nd largest state in America. Administratively Florida is divided into 410 cities and towns and 67 counties. The states population in 2013 was 19,552,860, according to the 2013 United States Census, making it the 4th most populous state in America. ...