RESULTS Profiles of Sl–Sa1/Sl–Sa2 B-cell switching in systemic and mucosal immune compartments Genomic DNA purified from peripheral blood lymphocytes (PBLs), spleen, and various mucosal tissues was subjected to nested PCR to amplify the Sm–Sa switch fragments that contain the recombination ...
Our results underline the importance of laboratory testing of bacteria in their native niche or in conditions that mimic their niche environment. The use of nutrient-rich culture media in general research practice will often hide phenomena that depend on the unique conditions in the body niches that...
resultsindicatedthatinnormalindividuals,onthesurfacesofhepatocytes(97).Althoughreceptor peripheral-bloodBcellsproduce,uponpoly-nonparenchymallivercellscanalsobindand clonalstimulationwithmitogensorEBVtrans-catabolizeIgA,hepatocytesappeartobemore formation,predominantlypIgA;theratioofactiveinthisprocess.Thisconclusionwas ...
Blood was collected from mice through saphenous vein bleeding. Fresh fecal pellets were collected from individual mice and solubilized in 50 g ml−1 of soybean trypsin inhibitor (Sigma-Aldrich, St Louis, MO) in sterile PBS (10× v/w) by con- tinual vortexing for 30 min at 4 °C and...
In general, IgA deficiency is often found in healthy blood donors with a defect in the differentiation of IgA cell-producing plasma B-cells but without any harm of such individuals [41]. The functions of IgA at the body sur- face are well known. IgA forms a barrier against pathogens by ...
This finding suggests that the plasmablasts cir- culating in the blood are not uniformly distributed along the gastrointestinal tract. It remains to be identified when and how, during antibody maturation process, the target specific- ity of mucosally secreted IgA changes. We also found that the ...
Blood cholesterol TG: Blood triglycerides HDL: High-density lipoprotein LDL: Low-density lipoprotein eGFR: Estimated glomerular filtration rate 24h-UTP: 24-hour urine protein M: Mesangial hypercellularity E: Endocapillary hypercellularity S: Segmental glomerulosclerosis T: Tubular atrophy/int...
At this time she was given a 20 ml test dose of blood group compatible plasma lacking IgA, which she tolerated well without ill effects. Case No. 4 (P.J.) A 43-yr-old man had had recurrent anemia since 1956 that had been treated with parenteral iron and blood transfusions. In 1970...
Results Study population A total of 434 cohort children were actively followed up for 1 year and the incidence of malaria (annual episodes per child) was determined. Of the 434 children, 2.5% (11/434) did not provide an adequate blood sample for subsequent analysis of DNA. The remaining 423...
Using data-driven expression-prioritized integration for complex traits (DEPICT) analysis26, we further prioritized 17 tissues and cell types at FDR < 0.05 based on our GWAS results, with the strongest enrichment in bone marrow cells, hematopoietic system, as well as blood and myeloid cells ...