which proved to be the base module of IFT-A at a resolution of 4.8 Å based on the gold-standard Fourier Shell Correlation (FSC) cut-off criterion of 0.143. A new round of particle picking was iterated for all datasets by using projections of this newly obtained density map. The new...
zvps15 wild-type and zvps15- R976Q mRNA were injected at the final concentration of 40 ng ml À 1. Materials and methods for yeast cells. The R998Q mutation was introduced into the hVPS15 cDNA by polymerase chain reaction (PCR) with Phusion High- Fidelity DNA polymerase (Thermo ...