Results: Our data show that both IFN α and IFN γ decreased the HDC mRNA and protein expression, though with dissimilar kinetics. IFN γ strongly suppresses the proliferation at 72 h, while IFN α has a more moderate effect. Conclusions: Since previously the inhibitory effect of histamine on...
We investigated the effects of pegylated (PEG)-IFN-alpha2b on alpha-fetoprotein (AFP) expression as demonstrated by protein and mRNA levels in six human hepatocellular carcinoma (HCC) cell lines. The number of KIM-1 cells in culture with PEG-IFN-alpha2b decreased between 24 amd 240 h, where...
This PMA-mediated inhibition of IFN-alpha/beta secretion is correlated with considerably reduced levels of IFN-alpha/beta mRNA, as determined by Northern blot analysis. Nuclear run-on assays further show that, at least in monocytes, PMA inhibits transcription of the IFN-alpha and -beta genes. ...
Viral mRNA and protein synthesis was clearly seen in virus-infected lung cells. A549 and HFL1 cells produced only small amounts of IFN-alpha/beta, whereas virus-infected macrophages produced type I IFN very efficiently. The kinetics of IFN-beta gene expression in A549 cells was rapid, as ...
Protein synthesis de novo is not required for the elevation of XD mRNA after IFN-alpha treatment, since cycloheximide does not block the induction. The ... M Terao,G Cazzaniga,P Ghezzi,... - 《Biochemical Journal》 被引量: 212发表: 1992年 Adjuvant Activity of Cytokines Although a number ...
IPEC-J2 were stimulated with IFN at indicated concentrations for 24 h in 24-well plates, and the mRNA of ISG15, OASL, MxA, IFITM1 or IFITM3 was measured by relative RT-qPCR. The results are presented as the mean ± SEM (N = 3–4). In addition, interferon-induced transmembrane protei...
The serum level of IL-6 and expression of IL-6 mRNA in hepatocytes from regenerating liver were investigated in the rat. The IL-6 level in the serum was not significantly different from that of a control group of rats submitted to an acu... S Sofia,A Casali,E Buscarini - 《Ital J ...
The presence of IL-2, IFN-gamma, and TNF alpha and the absence of IL-4 mRNA in samples of ITL indicate a pattern of cytokine production that most... PF Watson,AP Pickerill,R Davies,... - 《Journal of Clinical Endocrinology & Metabolism》 被引量: 292发表: 1994年 Effector Cell-Derived...
Boswellic acids, triterpenoids derived from the genus Boswellia (Burseraceae), are known for their anti-inflammatory and anti-tumor efficacy. Atopic dermatitis is a chronic, non-infectious inflammatory skin disease. However, the effects of α-boswellic a
1996: Single cell differential mRNA display for the detection of genes specific for T-cell lymphoma and Hodgkin and Reed-Sternberg cells of Hodgkins disease Annals of Hematology 73(Suppl 2): A127 Trümper, L.H.; Brady, G.; Bagg, A.; Gray, D.; Loke, S.L.; Griesser, H.; Wagman, ...