These results indicate that the augmentation of induction and/or accumulation of MHC mRNA by IFNs is not dependent on the de novo synthesis of protein, but the down-regulation of class II mRNA level by IFN-β is mediated by some newly synthesized protein(s)....
and rat cultured mast cells, RCMC) and mast cells from the rat body cavity were used to test the hypothesis that IFN-α/β and IFN-γ inhibit tumor necrosis factor α (TNF-α)-mediated cytotoxicity by depressing the steady-state levels of mRNA for TNF-α....
Results: Our data show that both IFN α and IFN γ decreased the HDC mRNA and protein expression, though with dissimilar kinetics. IFN γ strongly suppresses the proliferation at 72 h, while IFN α has a more moderate effect. Conclusions: Since previously the inhibitory effect of histamine on...
Abundance of IFN-alpha and IFN-gamma mRNA in blood of resistant and susceptible chickens infected with Marek's disease virus (MDV) or vaccinated with turke... The effects of the very virulent RB-1B strain of Marek's disease virus (MDV) and turkey herpesvirus (HVT), a vaccinal strain, ...
We investigated the effects of pegylated (PEG)-IFN-alpha2b on alpha-fetoprotein (AFP) expression as demonstrated by protein and mRNA levels in six human hepatocellular carcinoma (HCC) cell lines. The number of KIM-1 cells in culture with PEG-IFN-alpha2b decreased between 24 amd 240 h, where...
This PMA-mediated inhibition of IFN-alpha/beta secretion is correlated with considerably reduced levels of IFN-alpha/beta mRNA, as determined by Northern blot analysis. Nuclear run-on assays further show that, at least in monocytes, PMA inhibits transcription of the IFN-alpha and -beta genes. ...
Protein synthesis de novo is not required for the elevation of XD mRNA after IFN-alpha treatment, since cycloheximide does not block the induction. The ... M Terao,G Cazzaniga,P Ghezzi,... - 《Biochemical Journal》 被引量: 212发表: 1992年 Inhibition of Interferon Signaling by the Kaposi'...
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Antisense oligonucleotides complementary to Stat1 alpha mRNA were introduced in CH235-MG astroglioma cells by transient transfection; such treatment inhibited both constitutive and IFN-gamma-enhanced expression of Stat1 alpha. IFN-gamma-induced class II MHC expression was also inhibited in cells exposed ...
Figure 3. (A–E) Effects of α-boswellic acid on the TNF-α/IFN-γ-induced proinflammatory cytokines and chemokine mRNA expressions in HaCaT cells. (F–H) Effects of α-boswellic acid on involucrin, filaggrin, and loricrin mRNA expressions in TNF-α/IFN-γ-induced HaCaT cells. HaCaT cells...