The lysates were incubated with BSA-blocked Ni2 þ –NTA or GSH beads for 1 h followed by three washes with lysis buffer. Bound material was eluted in lysis buffer supplemented with 500 mM imidazole (Ni2 þ –NTA beads) or 30 mM reduced glutathione (GSH-beads). Large-scale ...
Are salivary stones painful? Is a blocked salivary gland dangerous? How to get rid of salivary stones naturally? Does eustachian tube dysfunction go away? Does the eardrum flap? Can a person have a cough reflex whilst their gag reflex is absent?
Galactostasis or milk stasis (uncomfortable milk build up in the udder often associated with such factors as: over-production of milk, small litter size, blocked milk ducts or the abrupt loss or removal of a suckling litter); Mastitis - infection of the breasts (usually painful); Fibroadenomat...