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The salinity, pH, and temperature of the samples were measured using a handheld refractom- eter (MASTER-S28α, ATAGO Co., Ltd., Tokyo, Japan), pH meter (EcoTestr pH1, Eutech Instruments, Thermo Fisher Scientific, Waltham, MA, USA), and thermometer (CENTER 300, CENTER Technology Corp.,...
qRT-PCR reactions were performed on an Applied Biosystems 7900 HT Fast Real-Time PCR System (Thermo Fisher Scientific, Schwerte, Germany) with the Fast Evagreen qPCR master mix (Biotrend, Cologne, Germany). The master mix was used in a 0.75× concentration. Further components of the reaction...