The PrimerQuest Tool helps to enable the design of basic and highly customized primers and probes for PCR and qPCR. Now that you understand the fundamentals of using this design tool, look for future articles from IDT about experiments requiring more complex assay design considerations, such as ...
Design PCR and qPCR assays intelligently for any species, with PrimerQuest. Fine-tune primer and probe design by adjusting the tool’s custom design parameters.
Custom PCR Primers ReadyMade Oligos CRISPR Gene Editing Guide RNAs Cas Enzymes HDR Reagents rhAmpSeq™ CRISPR Data Analysis System Gene Synthesis & Fragments Next Generation Sequencing Library Preparation Hybridization Capture Amplicon Sequencing
How to design primers and probes for PCR and qPCR Find everything you need to know about primer and probe design, from optimal GC content to annealing temperature. READ THE ARTICLE CRISPR Basics Handbook Level-up your gene editing workflow with insights into enzyme selection, guide design, HDR...
1) 打开IDT（Integrated DNA Technologies）官网，将CDS序列粘贴至如图位置，点击design assay 2) 跳转页面后勾选Exons 3) 下拉页面点击 design assays，稍等一会就会出现推荐的引物 （如图）二、Primer Bank 1) 进入NCBI搜索想要的基因后，记下其基因ID和相应来源 2) 进入Primer Bank官网 选择NCBI Gene ID以及相应...
a resuspension calculator and an application for analyzing oligo secondary structures. ‘OligoAnalyzer’ can be used to calculate the physical properties of any DNA or RNA sequence, whereas ‘PrimerQuest’ and ‘RealTime PCR’ simplify the design of PCR and qPCR primers and probes. Each optimized...
The xGen Normalase research workflow begins after NGS library adapter ligation, using either full-length indexed adapters or truncated adapters, where Normalase PCR primers are used to amplify the libraries to above the minimum threshold and condition the libraries for downstream Normalase enzymology....
LNA design Tool to design LNA modified probes and primers having specific duplex stability. ddRNAi design Design sequences for DNA-directed RNA interference. RNAi design Tool to design siRNA duplex oligomers. TriFECTa RNAi Kits Predesigned dicer-substrate RNAi duplexes, gene knockdown kits. An...
How to design primers and probes for PCR and qPCR Find everything you need to know about primer and probe design, from optimal GC content to annealing temperature. READ THE ARTICLE CRISPR Basics Handbook Level-up your gene editing workflow with insights into enzyme selection, guide design, HDR...
How to design primers and probes for PCR and qPCR Find everything you need to know about primer and probe design, from optimal GC content to annealing temperature. READ THE ARTICLE CRISPR Basics Handbook Level-up your gene editing workflow with insights into enzyme selection, guide design, HDR...