synthetic gRNA (sgRNA) [ , , ], Alt-R® A.s. Cas12a nuclease V3 and crRNA, ssODN synthesis (Ultramer DNA oligos), and electroporation enhancer buffers were from Integrated DNA Technologies (IDT) (Coralville, IA, USA). c provides the nucleotide sequences...
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InformationServices,IntegratedDNATechnologies,Inc.,1710CommercialPark,Coralville,IA52241,USA ReceivedJanuary31,2008;RevisedMarch27,2008;AcceptedApril3,2008 ABSTRACT DNAandRNAoligomersareusedinamyriadof diversebiologicalandbiochemicalexperiments. Theseoligonucleotidesaredesignedtohave ...
synthetic gRNA (sgRNA) [ , , ], Alt-R® A.s. Cas12a nuclease V3 and crRNA, ssODN synthesis (Ultramer DNA oligos), and electroporation enhancer buffers were from Integrated DNA Technologies (IDT) (Coralville, IA, USA). c provides the nucleotide sequences...