The positive IDH 1 (R132H) status of each case was previously determined with pyrosequencing and H09 immunohistochemistry on permanent sections. The FS control tissue of each case was immunostained with H09 antibody. Among 30 gliomas, 25 showed negative reactivity on FS control slides, whereas, ...
Moreover, the tool differentiates IDH1 R132H immunostain negative cases from cases without previous testing and provides separate scores. This tool might help to better triage brain tumor cases for additional molecular testing. Online-tool for predicting likelihood of IDH1/2 mutation in glioma For ...
This compari- son showed that the results were identical for all cases in terms of positive or negative. We used the scoring value of automated quantification for the analysis of our data. DNA sequencing DNA sequencing of IDH1 codon 132 and IDH2 codon 172 was performed in IDH1-R132H immuno...
Immunohistochemistryusing amonoclonal antibodyagainstIDH1-R132H, which accounts for more than 90% of glioma-associatedIDH1hotspot mutations, is now commonly used for mutation assessment ofIDH1mutation in FFPE tissue sections (Capper et al., 2010). In the case of IDH1-R132H-negativeimmunostaining, te...
Acta Neuropathologica Communications (2015) 3:4 DOI 10.1186/s40478-014-0180-0 RESEARCH Open Access Effective immuno-targeting of the IDH1 mutation R132H in a murine model of intracranial glioma Serena Pellegatta1, Lorella Valletta1, Cristina Corbetta1, Monica Patanè1, Ileana Zucca1, Federico ...
Using human cytokine arrays that query 36 proteins, we identified significant downregulation of ICAM-1/CD54 in IDH1R132H/WT gliomas, which was further confirmed by ELISA and immunoblotting analyses. ICAM1 gain-of-function studies revealed that ICAM1 downregulation in IDH1R132H/WT cells played a ...
e, Relative survival as detected by PicoGreen assay in IDH1-/- MiaPaCa-2 cells, transiently transfected with empty vector (EV), plasmid overexpressing catalytically active, or catalytically altered (R132H) IDH1, and treated with gemcitabine for 5 days under 5 mM glucose (relative glucose ...
The R132H-IDH1 expression may be present only in a fraction of tumour cells in some diffuse gliomas, therefore a negative result does not necessarily exclude a glial lesion as the concentration of immuno-positive diffuse astrocytomas ranges between 50% and 70% [14]. Thus, several sections of...
Abstract Mutations in isocitrate dehydrogenase (IDH) 1 and 2, originally discovered in 2008, occur in the vast majority of low-grade gliomas and secondary high-grade gliomas. These mutations, which occur early in gliomagenesis, change the function of the enzymes, causing them to produce 2-hydrox...
Our results suggest that the pathogenesis of multicentric gliomas is different from the mutant IDH1-R132H pathogenesis of lower-grade glioma and secondary glioblastomas. More studies are needed to confirm the molecular mechanisms underlying the pathogenesis of multicentric glioma.Karlowee V....