IDH Activity Assay Kit Isocitrate Dehydrogenase Activity Assay 异柠檬酸脱氢酶活性检测 基本信息 NACRES NA.84 General description【一般描述】 异柠檬酸脱氢酶 (IDH) 催化异柠檬酸转化为 α-酮戊二酸。在真核生物中,IDH 有三种同工酶,线粒体 IDH2 和 IDH3 以及细胞质/过氧化物酶体 IDH1。所有三个 IDH...
IDH Activity Assay Kit Isocitrate Dehydrogenase Activity Assay 异柠檬酸脱氢酶活性检测 基本信息 NACRES NA.84 General description【一般描述】 异柠檬酸脱氢酶 (IDH) 催化异柠檬酸转化为 α-酮戊二酸。在真核生物中,IDH 有三种同工酶,线粒体 IDH2 和 IDH3 以及细胞质/过氧化物酶体 IDH1。所有三个 IDH...
IDH Activity Assay Kit Isocitrate Dehydrogenase Activity Assay 异柠檬酸脱氢酶活性检测 基本信息 NACRES NA.84 General description【一般描述】 异柠檬酸脱氢酶 (IDH) 催化异柠檬酸转化为 α-酮戊二酸。在真核生物中,IDH 有三种同工酶,线粒体 IDH2 和 IDH3 以及细胞质/过氧化物酶体 IDH1。所有三个 IDH...
英文名称 IDH Activity Assay Kit 储存温度 -20°C储存 运输条件 超低温冰袋运输 产品介绍 异柠檬酸脱氢酶 (IDH) 催化异柠檬酸转化为 α-酮戊二酸。在真核生物中,IDH 有三种同工酶,线粒体 IDH2 和 IDH3 以及细胞质/过氧化物酶体 IDH1。所有三个 IDH 家族成员的酶活性均需要存在二价阳离子(Mg2 +...
在多种癌症,如成胶质细胞瘤、急性骨髓性白血病及直肠癌中,已发现存在IDH1及IDH2突变所导致的新生变体酶活性。这种新变体活性表现出了底物特异性的改变,从而导致α-酮戊二酸转化为2-羟戊二酸。IDH家族成员发生的突变还与内生软骨瘤病及马富西氏综合症相关。
IDH activity assay The activity of isocitrate dehydrogenase was determined by measuring the increase of absorbance at 340 nm generated by the conversion of NADP to NADPH at 25 oC. These assays were performed in total cell lysate, as well as in cytosol and mitochondrial fractions. The standar...
Cells were cultured with the indicated compounds or GL261 cell line supernatant, and the activity of the reporter was measured after 6 h using a Promega luciferase assay kit (E1500) following the manufacturer’s instructions. Luminescence was measured using a PHERAstar FS plate reader (BMG ...
We observed in this dataset as well a significant negative association between AC-like differentiation and cell cycle activity (Pearson’s r = −0.35, p = 4.15−5, t test); however, this association was significantly weaker in untreated relative to treated samples (Figure S5A), suggesting...
The assay continuously monitors the formation of NADPH as an increase in absorbance at 340 nm (36). When compared with WT IDH2, substitutions at Lys-256, Lys-263, and Lys-272 displayed no significant change in activity. Strikingly, activity of the K413Q mutant decreased by 20-fold, ...
Interestingly, this RNP can only successfully trap Wip1 when the LOC RNA is opened up by DHX15 helicase activity. Could the LOC–DHX15 RNP complex be responsible for sustained sequestering of Wip1 away from p65 and p38, thereby unleashing the coordinated activation of both p65 and p38 that ...