Transfection was performed using liposome nucleic acid transfection reagent (YEASEN). The cells were then incubated with these reagents and packaging plasmids for 48 h, and the resulting supernatant was collected. PANC-1 cells at approximately 30% density were cultured 24 h before infection and then...
Another approach would be to incorporate phenolics with o-diol functionality (e.g., methyl caffeate 5, caffeoylquinic acid 6, epicatechin 9, epigallocatechin 10, and epigallocatechin gallate 11). The presence of o-diols provides an intramolecular pathway to trap lignin quinone methide intermediates...
conjugate containing the first nucleic acid probe and the number of molecules of conjugate containing the second nucleic acid probe in the sample solution according to a scanning molecule counting method; and, a step for identifying polymorphism of the target nucleic acid based on the calculation ...
1. A conjugate comprising: (i) a cell-penetrating peptide (CPP), or analog thereof, comprising an amino acid sequence at least 90% identical over the entire length of any one of SEQ ID NOs:11-13, wherein the cell-penetrating peptide is not a full-length protein that occurs in nature,...
1. A method of making a treatment decision for a canine subject, comprising: determining whether a gene-truncation mutation in a mdr1-encoding sequence or a truncated P-gp encoded by the gene-truncation mutation in the mdr1-encoding sequence is present in the canine subject; and making a tr...
CONSTITUTION:The specific nucleic acid sequence is detected by the hybridization between DNA-DNA, RNA-RNA or DNA-RNA. The primary probe including the complementary sequence or the secondary probe which can conjugate specifically with the primary probe and the labeling material which can make self ...
conjugate containing the first nucleic acid probe and the number of molecules of conjugate containing the second nucleic acid probe in the sample solution according to a scanning molecule counting method; and, a step for identifying polymorphism of the target nucleic acid based on the calculation ...
Methods of identifying a patient having an altered immune status involve determining an immune status index for the patient and comparing it to the immune status index in healthy individuals. In gener
The present invention relates to methods for identifying amino acids in peptides. In one embodiment, the present invention contemplates labeling the N-terminal amino acid with a first label and labeli
The term 'hybridization' means any process by which a strand of nucleic acid binds with a complementary strand through base pairing. The term 'hybridization complex' refers to a complex formed between two nucleic acid sequences by virtue of the formation of hydrogen bonds between complementary base...