Background/Objectives The use of proxy measures such as the c difficile Lab ID event has been used to identify C difficile incidence and prevalence rates, and has been proposed as an efficient tool to perform s
The collected sam- ples were used for ssRNA-Seq and Real-Time Quantita- tive PCR (qRT-PCR) analysis. Glucose Tolerance Test (18 d): Following a 12-h fast, male AA broilers (approximately 540 ± 50 g in body weight) were randomly selected and divided into the experimental group...
We used a RT–PCR test that targets the N and orf1ab genes of SARS-CoV-2, a combination that is used routinely for diagnostic screening for SARS-CoV-2 infections in Rwanda. The standard protocol is to consider a test positive if PCR amplification produces an above-background fluorescence ...
The cDNA synthesis was performed using reverse transcription reaction kit (Vazyme) following the manufacturer’s instructions. Quantitative real-time PCR was carried out using the Hieff Universal qPCR SYBR Green Master Mix (UNICON, Yeasen Biotechnology, Shanghai, China). Primer sequences used for qRT...
Flow cytometry instruments are extensively used in the field of microbiology due to their ability to analyze and sort single cells in real time. These tools are capable of distinguishing between live and dead cells and can be coupled with any downstream analysis, thereby providing valuable insights...
and can be downloaded from https://deleteome.holstegelab.nl Our DeleteomeTools software was originally developed to identify gene products that are functionally associated with the nucleoporin NUP170. We used the data in the Deleteome to identify genes that, when deleted, alter the yeast transcr...
(RNaseH-). DNA polymerase I and RNase H were used to synthesize the second strand of cDNA. In the reaction buffer, dTTP was replaced by dUTP in the dNTPs mixture. The resulting product was purified by AMPure XP system, and the library quality was evaluated by Agilent Bioanalyzer 2100 ...
Alternatively, GRO-seq can be used to identify all expressed lncRNAs in a cellular context of interest. CRISPRi screen Here, we describe the design of gRNAs to target the transcription start sites (TSSs) of selected lncRNAs, using the GRO-Seq data generated in the first part of this ...
The PCR product for the Y-specific gene (210bp) was used as a positive biomarker for male fetii [17]. The simultaneous presence of 341 bp and 467 bp fragments of the bAML gene indicated a male fetus in contrast to only 467bp product in a female fetus. The limitation with the bAML ...
The PCR conditions were: 93°C for 5 min and then a variable number of cycles (26 to 34) at 93°C for 30 sec, 1 min at 55°C, and 1 min at 72°C. The PCR reaction was with a final step at 72°C for 10 min. Table 1 Primers used in this study Full size table Cloning ...