Transplantation of HSCs cultured in these artificial niches resulted in long-term blood reconstitution in vivo. These experiments provide an approach to identify stem cell niche signals based on single-cell fate analysis. Results Cell-state-specific gene expression signatures To characterize the gene ...
Genomic DNA from 19 tumor tissues and matched blood samples was extracted with the Biospecimen Extraction Resource of MD Anderson Cancer Center. Genomic DNA from matched blood samples were used as germline controls. 500 ng of DNA per sample was proceeded to library preparation using Roche KAPA l...
Ischemic heart disease is one of the leading causes of mortality worldwide, and thus calls for development of more effective therapeutic strategies. This study aimed to identify potential therapeutic targets for coronary heart disease (CHD) and myocardia
CD1b-Ac2SGL tetramers are multimers of CD1 proteins that are fluorescently labeled and loaded with Ac2SGL to take advantage of high avidity to bind polyclonal T cells, even when present in low numbers of total blood cells. This powerful technique can be used to detect antigen-specific T ...
(LRP6) interactions specific to engineered capsids with enhanced blood-brain barrier crossing in non-human primates after intravenous administration. The unbiased cell microarray screening approach also allows us to identify off-target tissue binding interactions of engineered brain-enriched AAV capsids ...
Google Scholar 3. Young W: Spinal cord regeneration.Cell Transplant2014;23:573–611. Go to Citation Crossref PubMed Google Scholar 4. Diaz-Arrastia R, Kochanek PM, Bergold P,et al.: Pharmacotherapy of traumatic brain injury: state of the science and the road forward: report of the Depart...
The integrative score improves prediction of drug sensitivity for the known drug targets of these compounds compared to each data type alone. The gene activity scores are also used to cluster colorectal cancer cell lines. Two subtypes of CRCs were found and potential cancer drivers and therapeutic ...
In more caudal structures, such as the intestine and lower urinary tract, our Sox10-cre BAC transgene appears to be more efficient in labeling neural crest-derived cell types than Wnt1-cre. The analysis reveals consistent expression of Sox10 in non-neural crest derived glandular epithelium, ...
Each microchamber features a different cell-id tag sequence in the DNA probes immobilized on the beads. (b) Fluorescence microscopy image of the VFAC with trapped cells, where small white dots indicate individual cells. (c) Structure of DNA probes on the beads for mRNA trapping and 1st cDNA...
Moreover, the interaction between immune and non-immune cells within the TME can influence the effectiveness of immune responses, leading to varied treatment outcomes among patients. Single-cell analyzes in cancer research utilize a wide array of advanced techniques aimed at understanding the ...