Genomic DNA from 19 tumor tissues and matched blood samples was extracted with the Biospecimen Extraction Resource of MD Anderson Cancer Center. Genomic DNA from matched blood samples were used as germline controls. 500 ng of DNA per sample was proceeded to library preparation using Roche KAPA l...
Transplantation of HSCs cultured in these artificial niches resulted in long-term blood reconstitution in vivo. These experiments provide an approach to identify stem cell niche signals based on single-cell fate analysis. Results Cell-state-specific gene expression signatures To characterize the gene ...
These data have revealed that the presence of a hydroxyl group, the number of C-methyl substituents on the acyl chains, the configuration of the chiral centers, and the respective localization of the two different acyl chains on the sugar moiety govern TCR recognition and T cell activation (...
Further analyses, including enrichment analysis, single-cell analysis, MR analysis of cardiovascular risk factors, phenome-wide Mendelian Randomization (Phe-MR), and protein-protein interaction (PPI) network construction were conducted to verify the roles of selected causal proteins. Results Thirteen ...
(LRP6) interactions specific to engineered capsids with enhanced blood-brain barrier crossing in non-human primates after intravenous administration. The unbiased cell microarray screening approach also allows us to identify off-target tissue binding interactions of engineered brain-enriched AAV capsids ...
Ramón y Cajal S:Degeneration and Regeneration of the Nervous System. Oxford University Press, New York, 1928. Go to Citation Google Scholar 2. Committee on Spinal Cord Injury:Spinal Cord Injury: Progress, Promise, and Priorities. National Academies Press, Washington, D.C., 2005. ...
An oncogene is a gene that has the potential to cause cancer and it is often mutated in tumor cells. A tumor suppressor is a gene that protects a cell from cancer. Oncogenes present gain of function alterations (GoF), while inactivated tumor suppressor genes present loss of function (LoF)...
The cell suspension is then placed in a magnetic field that enriches for epithelial cells that bind to the anti-EPCAM antibody-coated ferro particles. The isolated cells are then incubated with the nucleic acid dye 4′,6-diamidino-2-phenylindole- and allophycocyanin-labeled anti-CD45 monoclonal ...
Each microchamber features a different cell-id tag sequence in the DNA probes immobilized on the beads. (b) Fluorescence microscopy image of the VFAC with trapped cells, where small white dots indicate individual cells. (c) Structure of DNA probes on the beads for mRNA trapping and 1st cDNA...
To systematically define molecular features in human tumor cells that determine their degree of sensitivity to human allogeneic natural killer (NK) cells, we quantified the NK cell responsiveness of hundreds of molecularly annotated ‘DNA-barcoded’ solid tumor cell lines in multiplexed format and appl...