To identify potential genetic modifiers of DPR protein production, we established stable reporter cell lines suitable for unbiased genome-wide CRISPR-Cas9 screening. We fused (GGGGCC)70 repeats (including the preceding intronic sequences) with EGFP in the GA frame (Figure 1A) and engineered this re...
Alternative splicing is crucial for cancer progression and can be targeted pharmacologically, yet identifying driver exons genome-wide remains challenging. We propose identifying such exons by associating statistically gene-level cancer dependencies from
However, you’ll likely need a magnifying glass to see these. Don’t have a magnifying glass on hand? If your phone has a decent camera, use the macro-mode to take a picture and zoom in. What you want to look for is the cross-eyes. Yep, this shovel-faced pest is also cross-eyed...
12 This sequence conservation suggests that inhibitors targeting nsp14 in SARS-CoV-2 could potentially also inhibit nsp14 MTase activity in other coronaviruses, which may have implications for the development of broad-spectrum antiviral drugs.13 In this study, we developed a computational pipeline ...
For functional annotation, all sequences were subjected to gene ontology (GO) analysis in Blast2GO, with 12,714 (56%) of the 22,875 contigs sharing significant similarity to proteins with assigned molecular functions in the GO d atabase43, whereas 44% of the total transcripts did not ...
The primary plant cell wall is a complex mixture of polysaccharides and proteins encasing living plant cells. Among these polysaccharides, cellulose is the most abundant and useful biopolymer present on earth. These polysaccharides also represent a rich
(Fig.1b). Restricting the database to polypeptide sequences originating from highly expressed genes should, on the one hand, improve the accuracy of MS-based non-canonical peptide identification, while on the other hand, lead to the potential loss of peptides encoded by lower-expressing ...
concentrations with no clear trends for different technical approaches: ricin detection was achieved at similar success rates (50%–63%) using immunological, MS-based and functional approaches, with a tendency that MS-based and functional methods are somewhat better suited for the correct assignment ...