We examined the isotopic signatures (δ13C, δ15N) of adult body feathers from southern giant petrels Macronectes giganteus collected at two breeding colonies in Antarctica (Potter Peninsula and Cape Geddes) and one in southern Patagonia (Observatorio Island), as well as in whole blood collected...
The resulting colonies were scraped and harvested in bulk at a coverage of more than 500 colonies per crRNA. The library plasmids were extracted using the NucleoBond Xtra Maxi EF Kit (Macherey-Nagel) to avoid endotoxin contamination. Library quality was assessed by next-generation sequencing. Pool...
Colonies were then grown overnight in liquid LB medium supplemented with the same antibiotics, 20 μM acetosyringone, and 10 mM 2-(N-morpholino)ethanesulfonic acid (MES, pH 5.6). When necessary, glycerol stocks of the strains in liquid culture were done by adding glycerol to a final ...
male” the person asking for help becomes unhinged. “You’resupposed to be the expert, but evenIcan see it’s not a honey bee!” For some species,
Single colonies were picked and expanded in six-well plates. Protein expression levels in each colony were determined by immunoblotting. Purification of protein complexes and mass spectrometry. AP-MS experiments were performed as previously described12. For protein purification, HEK293 cell lines stably...
After treatment of 10 days, colonies were fixed by ice-cold methanol and stained by crystal violet. Cell colonies were measured by ImageJ. The experiment was conducted with triplicates per group. Cell apoptosis and cell cycle assays Cell apoptosis was evaluated using FITC Annexin V Apoptosis ...
A unique, protective cell envelope contributes to the broad drug resistance of the nosocomial pathogen Acinetobacter baumannii. Here we use transposon insertion sequencing to identify A. baumannii mutants displaying altered susceptibility to a panel of diverse antibiotics. By examining mutants with antibioti...
Once colonies grew to confluency the resultant GFP positive cells were sorted by FACS. Plasmid stable lines were maintained in MEM with 400 mg/mL of Geneticin where media was changed every two days. Human pluripotent stem cell (hPSC)-derived cerebral organoids were grown at 37 °C with 5% ...
One hundred percent of the colonies from the ∆advA/pMS88-advA strain retained the plasmid (18/18 retaining KanR); in contrast, pMS88 was lost from a large fraction of the parallel cultured WT control strain (6/18 colonies retaining KanR). In addition, ∆advA/pMS88-advA showed reduced...
Using the original four Yamanaka’s factors plus small hairpin TP53 plasmid, we have generated iPSC-like clones. To identify the possible CSC clones, we have reduced the number of cells that remain competent after tumor formation, as determined by the transplantation of colonies of xenograft-...