The proteins were recovered from the bands in two or three split fragments per lane and each fragment was independently subjected to further MS analysis (performed by Tsinghua University Phoenix Center using LTQ-Orbitrap Velos Mass Spectrometer). MS raw results and processed MiST results are ...
simplified model on shorter sequences with fewer parameters. Second, we empirically avoided overfitting by applying the L1-regularization (coefficient = 0.6) in the convolutional layers and the dropout strategy (see “Methods”) in the hidden layer during model training. Both of these factors m...
Paspalum notatum exhibits both sexual and apomictic cytotypes and, thus, is considered a good model for studies of apomixis because it facilitates comparative approaches. In this work, transcriptome sequencing was used to compare contrasting P. notatum c
In contrast, the number of heterozygous markers on each autosome is expected to be similar between the two parents. In a comparison of the two sex-specific maps, if one linkage group has a strong difference in marker number between the male and female maps, that linkage group is likely to...
All libraries were analysed for adapter dimers, size distribution and concentration on a Fragment Analyzer System using the DNF-474 HS NGS Fragment kit (1–6000 bp) (Agilent). After pooling the libraries in an equimolar ratio, the concentration and the size distribution of the lane mix was ...
Sequenced reads from scRNA-seq libraries were demultiplexed, aligned to the mm10 mouse reference, barcode processed, and Unique Molecular Identifier (UMI) counted using the 10X Genomics Cell Ranger (v2.0.1) pipeline46. Estimated number of cells captured per sample were between 1296–3290 with 34...
on a single lane of a HiSeq4000 (Illumina) using 50 bp single-end (SE) reads with 10% PhiX spike-in as previously described [7]. For sgRNA challenge screens in BC-2, sequencing was performed on a single flow-cell of a NextSeq 500 (Illumina) using 75 bp SE reads with 10% ...
Sequences generated from each lane were processed using proprietary DArT analytical pipelines. In the primary pipeline, the fastq files were first processed to filter away poor-quality sequences, applying more stringent selection criteria to the barcode region compared to the rest of the sequence. ...
PCR, followed by sequencing on Illumina Hiseq2500. The sequencing (single read) was run for 78 cycles. Sequences generated from each lane were processed using proprietary DArT analytical pipelines. In the primary pipeline, the fastq files were first processed to filter away poor-quality sequences...
PCR, followed by sequencing on Illumina Hiseq2500. The sequencing (single read) was run for 78 cycles. Sequences generated from each lane were processed using proprietary DArT analytical pipelines. In the primary pipeline, the fastq files were first processed to filter away poor-quality sequences...