Ubiquitinated proteins Abstract: Objective To identify ubiquitinated proteins from complex human multiple myeloma (MM) U266 cells,a malignant disorder of differentiated human B cells.Methods Employing a globally proteomic strategy combining of immunoprecipitation,LC-MS/MS and SCX-LC-MS analysis to identifi...
Identification of ubiquitinated proteins from human multiple myeloma U266 cells by proteomics. Biomed Environ Sci. 2011;24(4):422–30.Jia H, Liu C, Ge F, Xiao C, Lu C, Wang T, He Q: Identification of ubiquitinated proteins from human multiple myeloma U266 cells by proteomics. Biomed ...
FIGURE 1. In vivo interaction of RBCK1 with ubiquitinated proteins and self-ubiquitination activity of RBCK1. A, molecular organization of RBCK1. Amino acid residue numbers of the start and end of each motif are indicated. Ubl, ubiquitin-like; CC, coiled-coil; NZF, novel zinc finger; RING...
Identification of polyubiquitinated proteins binding to UBA domain of hFAF1.JaeJin, LeeYoung Mee, KimJaeho, JeongDuk, Soo BaeKongJoo, Lee
Ubiquitin-associated protein 1 (encoded byUBAP1) constitutes a subunit of mammalian endosomal sorting complex required for transport I (ESCRT-I) and is involved in endosomal dynamics in neurons. Furthermore, it is involved in proteasomal degradation of ubiquitinated proteins. Both cellular pathways hav...
click chemistry for the generation of biotinylated nonhydrolyzable diubiquitin baits, (ii) in vitro affinity purification of ubiquitin interactors, (iii) on-bead interactor digestion, (iv) liquid chromatography (LC)–MS/MS analysis and (v) data analysis to identify differentially enriched proteins. ...
USP13 is a well-characterized member of the USP family, which regulates diverse cellular functions by cleaving ubiquitin from ubiquitinated protein substrates. However, existing studies indicate that USP13 has no detectable hydrolytic activity in vitro. This finding implies that USP13 likely has ...
1e, f). Next, the effect of BBR and bortezomib on the proteasome activity and ubiquitinated protein level was compared in MM cells. This analysis showed that bortezomib induced a marked increase in ubiquitylated proteins than BBR, whereas a modest increase in polyubiquitylation was observed in ...
The identification and characterization of genes and proteins that are either cell-type specific or exhibit an otherwise spatially or temporally restricted expression pattern is a crucial step toward understanding the complex interactions between the different cell types in an organism. We demonstrate here...
et al. A comprehensive method for detecting ubiquitinated substrates using TR-TUBE. Proc. Natl Acad. Sci. USA 112, 4630–4635 (2015). Article PubMed PubMed Central CAS Google Scholar Sarraf, S. A. et al. Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial ...