Upon entering a permissive host cell, the enterovirus genome first serves as a template for translation, pro- ducing the viral polyprotein, and then becomes a template for replication of the minus strand. The 5′ UTR is fun- damentally required for these functions by interacting with ...
the New-Dehli metallo β-lactamase. CIM-1 and NDM-1 revealed low sequence identity (33.5%). However, some level of similarity was observed in the region of loops 3 and 10, which have been identified as important for substrate binding31,32(Fig.1a). Assessment of known conserved...
Newborn Screening, Metabolism & Genetics Unit, Human Genetics Department, National Institute of Health Doutor Ricardo Jorge, 4000-055 Porto, Portugal 3 Center for the Study of Animal Science, CECA-ICETA, University of Porto, 4051-401 Porto, Portugal ...
2.2. Cloning of the Genes Encoding Turtle Interleukin-1β (IL-1β) and Caspase-1 A partial fragment of the tIL-1β gene was amplified using the degenerate primer pair tIL-1-dF and -dR (Table S1). Synthesized cDNA from spleen was used as a template in PCR reaction with a denaturation...