Label-free sRANKL protein in this study was obtained by removing His6-sumo tag from fusion protein His6-sumo-sRANKL. The DNA sequence encoding sRNAKL was inserted downstream the His6-sumo tag of a modified pET20b (+) plasmid. Single point mutations of sRANKL (K182A, R192A, W194A, ...
This is even more evident in the case of the combination of state-of-the-art spectrometers with high resolving power and powerful bioinformatic tools, that made very popular the use of “label-free” quantification methods (LFQ) as an alternative to stable isotope labeling strategies.68., 69....
The PDPN+CD146−CD73+CD164+ cells had undergone multiple cycles of division as evidenced by log-fold reduced DiD-label on flow cytometry (Figure S2B). We then sorted DiDlo cells from the primary graft for an in vitro serial colony formation assay and a serial transplantation assay under ...
Briefly, approximately 10 × 106 cells were lysed in ice-cold polysome buffer (20 mM Tris pH 7.6, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT (Scientific Laboratory Supplies Ltd NAT1068), 100 µg/mL cycloheximide) supplemented with 1% v/v Triton X-100 and 25 U/mL Turbo...
TNB585.001: a multicenter, phase 1, open-label, dose-escalation and expansion study of tnb-585, a bispecific T-cell engager targeting PSMA in subjects with metastatic castrate resistant prostate cancer. Clin Oncol. 2021;39:TPS5092-TPS. Markowski MC, Kilari D, Eisenberger MA, McKay RR, Drei...
Two days before sample collection, CTB-555, an anterograde tracer, was intravitreally injected into eyes to label regenerating axons. Optic nerves and retinas were collected two weeks after ONC. B Representative images of optic nerve sections transduced with AAV2 vectors and labeled with CTB-555....
After then, the samples were mixed with 100 μl PicoGreen dye (Molecular Probe, Waltham, MA, USA; 200-fold diluted in 10 mM Tris–HCl, pH 8.0, and 10 mM EDTA) to label DNA, then incubated the mixture of samples and PicoGreen dye for 2 to 5 min, and finally, fluorescence (...
2: 1 3: 1 Heat Shrink Wire Identification Marker Sleeves Cable Label US$0.10-0.13 200 Meters (MOQ) PE material Heat Shrink Sleeving Electrical Cable Marker Identification Sleeve US$0.10-0.13 200 Meters (MOQ) Halogen Free Wire Identification Heat Shrink Cable...
If DNA flowers were employed as a label, the partially complementary strands that carry it were incubated in 10 mM MgCl2 for 2 h at ambient temperature, and subsequently, Amicon filtration was performed as described above. The asymmetry of the nanobait design allows for the unambiguous ...
Because each GPI-anchored protein has a single anchor, the level of radiolabel is proportional to the number of molecules of each protein. Remarkably the 11 identified GPI-anchored proteins constitute ∼94% of the GPI-anchored schizont proteome (Fig. 4). Hence even when considered together ...